Synthesis of high-molecular-weight poly([r]-(-)-3-hydroxybutyrate) in transgenic Arabidopsis thaliana plant cells
| Autor: | Yves Poirier, Michael Matthew Satkowski, Lee Arnold Schechtman, Chris Somerville, Isao Noda |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Magnetic Resonance Spectroscopy
Macromolecular Substances Polyesters Chemical structure Size-exclusion chromatography Arabidopsis Hydroxybutyrates macromolecular substances Biochemistry Gas Chromatography-Mass Spectrometry Polyhydroxyalkanoates Polyhydroxybutyrate Differential scanning calorimetry X-Ray Diffraction Structural Biology Organic chemistry Alcaligenes Molecular Biology Molecular Structure biology Chemistry technology industry and agriculture food and beverages General Medicine Nuclear magnetic resonance spectroscopy Plants Genetically Modified biology.organism_classification Molecular Weight Genes Bacterial Molar mass distribution lipids (amino acids peptides and proteins) Bacteria |
| Zdroj: | International Journal of Biological Macromolecules. 17:7-12 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/0141-8130(95)93511-u |
| Popis: | High-molecular-weight poly([R]-(-)-3-hydroxybutyrate) (PHB), a biodegradable thermoplastic, was produced from a suspension culture of transgenic Arabidopsis thaliana plant cells expressing two genes from the bacterium Alcaligenes eutrophus involved in the synthesis of PHB. The molecular structure of the plant-produced polymer was analysed by gas chromatography, mass spectrometry, proton nuclear magnetic resonance spectroscopy, infra-red spectroscopy, spectropolarimetry, differential scanning calorimetry, X-ray diffraction and size exclusion chromatography. The results indicate that the polymer from transgenic plants appears to have a chemical structure identical to that of PHB produced by bacteria. However, the molecular weight distribution of the plant-produced PHB was much broader than that of typical bacterial PHB. |
| Databáze: | OpenAIRE |
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