Cytochemical differentiation of the axon membrane in A- and C-fibres
| Autor: | S G Waxman, D C Quick |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Pathology
medicine.medical_specialty Guinea Pigs Neurilemma Biology Nerve Fibers Myelinated Cell membrane Nerve Fibers Ranvier's Nodes medicine Animals Cell Membrane Histological Techniques Sciatic Nerve Axons Axolemma Rats Staining Psychiatry and Mental health Membrane medicine.anatomical_structure nervous system Cytoplasm Biophysics Surgery Neurology (clinical) Sciatic nerve NODAL Research Article |
| Zdroj: | Journal of Neurology, Neurosurgery & Psychiatry. 40:379-385 |
| ISSN: | 0022-3050 |
| DOI: | 10.1136/jnnp.40.4.379 |
| Popis: | Guinea pig and rat sciatic nerves were fixed with cacodylate-buffered aldehydes and OsO4, and were stained with ferric ion and ferrocyanide. Cytoplasmic surfaces of the non-myelinated nodal axon membrane of A-fibres display distinct electron-dense aggregates of stain. These aggregates were not observed in association with the paranodal or internodal axolemma. The membranes of C-fibres exhibit no staining under these conditions. Thus, the nodal axolemma of normal myelinated fibres is structurally distinct from both the myelinated internodal membrane, and from the axolemma of C-fibres. The ferric ion-ferrocyanide technique may provide a method for marking axonal membrane with normal nodal properties. |
| Databáze: | OpenAIRE |
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