Correlation of polypeptide N-acetylgalactosamine transferases-3 and -6 to different stages of endometriosis
| Autor: | Kaihong Xu, Jun Lin, Xiaomin Xu, Xiangwei Fei, Junyan Ma, Yang Qu, Caiyun Zhou |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
medicine.medical_specialty media_common.quotation_subject Blotting Western Endometriosis Endometrium Real-Time Polymerase Chain Reaction 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine parasitic diseases medicine Humans Clinical significance RNA Messenger Menstrual cycle Menstrual Cycle media_common Messenger RNA 030219 obstetrics & reproductive medicine business.industry Obstetrics and Gynecology General Medicine Middle Aged medicine.disease Immunohistochemistry carbohydrates (lipids) Blot medicine.anatomical_structure Endocrinology chemistry 030220 oncology & carcinogenesis Galactosamine N-Acetylgalactosaminyltransferases lipids (amino acids peptides and proteins) Female business |
| Zdroj: | Archives of gynecology and obstetrics. 295(6) |
| ISSN: | 1432-0711 |
| Popis: | To investigate the expression patterns of N-acetyl galactosamine transferases (GalNAc-Ts)-3 and GalNAc-T6 in clinicopathologically characterized endometriosis (EMS), and to explore their clinical significance.Ectopic and eutopic endometrial tissue samples were obtained and confirmed with CD-10 immunohistochemistry in patients with EMS (n = 12), whereas normal control endometrium was obtained from patients with uterine septum (n = 12). The mRNA and protein levels of GalNAc-T3 and GalNAc-T6 were detected in these samples using quantitative real-time PCR, immunohistochemistry, and western blotting.GalNAc-T3 and GalNAc-T6 were expressed in the endometrium of all groups, with no significant changes observed during the menstrual cycle. The expression of GalNAc-T3 and GalNAc-T6 in ectopic endometrium was significantly lower than that in eutopic (P 0.05) or control endometrium (P 0.05), whereas there were no significant differences (P 0.05) between eutopic and control endometria. Furthermore, the expression of GalNAc-T3 and GalNAc-T6 was significantly lower in patients with stage III/IV EMS compared to patients with stage I/II (P 0.05).Both GalNAc-T3 and GalNAc-T6 expression levels were downregulated in ectopic endometrium, which may increase the adhesion and invasion of endometrial cells and contribute to the development of EMS. Moreover, we found a strong correlation between the expression of GalNAc-T3 and GalNAc-T6 and different stages of EMS. |
| Databáze: | OpenAIRE |
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