The application of microwave denaturation in comparative genomic hybridization
| Autor: | M. Jakobs, Marcel M.A.M. Mannens, A. Vink, Rosalyn Slater, Marja Steenman, A. Dietrich, M. Hermsen, M. de Meulemeester |
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| Přispěvatelé: | Other departments |
| Rok vydání: | 1996 |
| Předmět: |
Genome
Hybridization probe RNA Chromosome Reproducibility of Results Karyotype DNA Neoplasm Biology Nucleic Acid Denaturation Applied Microbiology and Biotechnology Molecular biology Wilms Tumor Genetics Nucleic acid Humans Denaturation (biochemistry) Metaphase chromosome Microwaves In Situ Hybridization Fluorescence Comparative genomic hybridization |
| Zdroj: | Genetic analysis, 13(5), 129-133. Elsevier BV |
| ISSN: | 1050-3862 |
| Popis: | Comparative genomic hybridization (CGH) is a powerful tool for analyzing unbalanced chromosomal rearrangements in a variety of tissues. However, the reproducibility of the technique is poor. We have developed an alternative protocol involving microwave denaturation of the metaphase chromosome preparations prior to the hybridization step. The advantage of this method for CGH is the retention of the morphology of the chromosomes and hence an improved chromosome banding pattern. Furthermore, it results in a cansistently strong hybridization which is not dependent on the batch of lymphocytes used to obtain the metaphase chromosome spreads. This procedure has also proved to be applicable to nucleic acid hybridizations in general. The protocol, its application and the results of this method in CGH is discussed. Furthermore preliminary results of this method in paint and DNA probe hybridizations to chromosome spreads and to RNA in tissue sections are presented. |
| Databáze: | OpenAIRE |
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