Revised rat multi-organ carcinogenesis bioassay for whole-body detection of chemopreventive agents: modifying potential of S-methylcysteine
| Autor: | Kenichiro Doi, Hideki Wanibuchi, Shoji Fukushima, Jun Shen, Makoto Mitsuhashi, Elsayed I. Salim, Shinzoh Kudoh, Kazuto Hirata, Min Wei |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Alkylating Agents Cancer Research Pathology medicine.medical_specialty Colon medicine.disease_cause Chemoprevention chemistry.chemical_compound medicine Animals Bioassay Diethylnitrosamine Cysteine Lung Carcinogen Glutathione Transferase Urinary bladder S-methylcysteine Neoplasms Experimental Multi organ Rats Inbred F344 1 2-Dimethylhydrazine Rats medicine.anatomical_structure Liver Oncology chemistry Organ Specificity Nitrosamine Carcinogens Cancer research Biological Assay Drug Screening Assays Antitumor Whole body Carcinogenesis |
| Zdroj: | Cancer Letters. 206:15-26 |
| ISSN: | 0304-3835 |
| Popis: | The DMBDD rat multi-organ carcinogenesis model based on two-stage carcinogenesis theory was revised to make more suitable assay system for detecting chemopreventive effects of chemical substances by increasing the doses of two carcinogens, 1,2-dimethylhydrazine dihydrochloride (DMH) and N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN). The revised bioassay resulted in increasing preneoplastic or neoplastic lesions in the colon, urinary bladder and liver. S-Methylcysteine (SMC), a water-soluble organosulfur compound, was used as a test chemical in the new initiation regimen. Though SMC did not express clear-cut inhibitory effects in tumor levels, it showed modifying effects on the development of lung hyperplastic and colon preneoplastic lesions. In conclusion, the present model featuring high yields of preneoplastic and neoplastic lesions with low mortality in a short period (30 weeks), might be suitable for testing the efficacy of possible chemopreventive chemicals at the whole-body level. |
| Databáze: | OpenAIRE |
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