Decreased synovial fluid pituitary adenylate cyclase-activating polypeptide (PACAP) levels may reflect disease severity in post-traumatic knee osteoarthritis after anterior cruciate ligament injury

Autor: Bing-Yin Sun, Zheng-Ping Sun, Wei-Tao Huang, Shao-Peng Wu, Zu-Cai Pang
Rok vydání: 2018
Předmět:
Adult
Male
medicine.medical_specialty
Physiology
Anterior cruciate ligament
Interleukin-1beta
030209 endocrinology & metabolism
Inflammation
Osteoarthritis
Biochemistry
Gastroenterology
Severity of Illness Index
03 medical and health sciences
Cellular and Molecular Neuroscience
Young Adult
0302 clinical medicine
Endocrinology
Internal medicine
Synovial Fluid
medicine
Adjuvant therapy
Synovial fluid
Humans
Receiver operating characteristic
business.industry
Tumor Necrosis Factor-alpha
Cartilage
Anterior Cruciate Ligament Injuries
Osteoarthritis
Knee
musculoskeletal system
medicine.disease
ACL injury
medicine.anatomical_structure
Pituitary Adenylate Cyclase-Activating Polypeptide
Female
medicine.symptom
business
hormones
hormone substitutes
and hormone antagonists
030217 neurology & neurosurgery
Biomarkers
Zdroj: Peptides. 116
ISSN: 1873-5169
Popis: Background It has been demonstrated that anterior cruciate ligament (ACL) injury-induced cartilage degeneration is the key risk factor for post-traumatic knee osteoarthritis (PTKOA).Pituitary adenylate cyclase-activating polypeptide (PACAP), a common neuropeptide exerting a wide spectrum of functions, has been proved to inhibit inflammation and prevent cartilage degeneration. Objective The current study was performed to investigate circulating and synovial fluid PACAP concentrations in ACL injury patients to determine their relationship with the disease progression of the severity of post-traumatic knee osteoarthritis (PTKOA). Methods 72 ACL injury patients receiving arthroscopical examination and surgery were enrolled in the study. Meanwhile, 60 gender-and-age non-traumatic patellar dislocation patients were enrolled as controls. The VAS score, Lysholm Score and International Knee Documentation Committee (IKDC) score were all recorded to evaluate the clinical severity. Serum and synovial fluid (SF) PACAP levels were investigated by enzyme-linked immunosorbent assay (ELISA).The IL-1β and TNF-α levels were also investigated. The degree of meniscus injury was assessed by MR imaging. The modified Mankin score was recorded to examine the cartilage histopathological alternations. Receiver operating characteristic (ROC) curve was performed to discuss the diagnostic value of PACAP levels for the prediction of the radiographic grading in comparison with IL-1β and TNF-α. Results Serum PACAP levels between PTKOA patients and patellar dislocation did not reach significant differences. However, SF PACAP levels were significantly lower in PTKOA patients than controls. In addition, SF PACAP levels were negatively associated with MRI imaging grade for meniscus injury and VAS score, and were positively associated with Lysholm and IKDC scores. In addition, SF PACAP levels were negatively related to Mankin score as well as the expressions of IL-1β and TNF-α. ROC analysis curve showed that attenuated PACAP may serve as a favorable marker for the diagnosis of MRI for meniscus injury. Conclusions SF PACAP concentrations showed an independent and negative association with disease severity in PTKOA following ACL injury. Local treatment with PACAP may act as a possible adjuvant therapy for delaying the process of PTKOA.
Databáze: OpenAIRE
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