Regulation of biosynthesis of secondary metabolites
| Autor: | Zdenko Vaněk, Z. Hošťálek, V. Jechová |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Malates
Streptomyces aureofaciens Chemical Fractionation Microbiology Malate dehydrogenase Divalent Metal chemistry.chemical_compound Biosynthesis Malate Dehydrogenase Magnesium Pyruvates Mycelium chemistry.chemical_classification Manganese biology Malate dehydrogenase (decarboxylating) General Medicine Hydrogen-Ion Concentration biology.organism_classification Enzyme chemistry Biochemistry Ammonium Sulfate visual_art visual_art.visual_art_medium |
| Zdroj: | Folia Microbiologica. 20:137-141 |
| ISSN: | 1874-9356 0015-5632 |
| DOI: | 10.1007/bf02876770 |
| Popis: | The process of isolation and purification of malate dehydrogenase (decarboxylating) (EC 1.1.1.40) from the mycelium of the actinomycete Streptomyces aureofaciens has been worked out. The enzyme was purified 35 fold. The kinetic characters of the purified enzyme are very similar to the figures for malate dehydrogenase (decarboxylating) from other sources. Km for L-malate = 2.1 X 10(-3)M, Km for NADP = 4.6 X 10(-5)M (at pH 7.4). The reaction requires metal divalent ions, Mn2+ being more effective than Mg2+. The enzyme reaches its maximal activity at pH 8.75. |
| Databáze: | OpenAIRE |
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