Zika virus infection of pregnant rats and associated neurological consequences in the offspring
| Autor: | Mark S. Parcells, Morgan L. Sherer, Jaclyn M. Schwarz, Erin M. Brannick, Pragyan Khanal, Gwen Talham |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male RNA viruses Microcephaly Physiology Gene Expression Apoptosis Pathology and Laboratory Medicine Hippocampus Zika virus Body Temperature Pathogenesis Rats Sprague-Dawley 0302 clinical medicine Pregnancy Animal Cells Chlorocebus aethiops Medicine and Health Sciences Medicine Materials Sickness behavior Cerebral Cortex Multidisciplinary biology Cell Death Zika Virus Infection Brain 3. Good health Solutions Flavivirus Physiological Parameters Medical Microbiology Cell Processes Prenatal Exposure Delayed Effects Viral Pathogens Viruses Physical Sciences Female Microglia Pathogens Cellular Types Anatomy Research Article Offspring Diluents Science Materials Science Glial Cells Research and Analysis Methods Microbiology 03 medical and health sciences Immune system Seizures Genetics Animals Animal Models of Disease Vero Cells Microbial Pathogens Microglial Cells Biology and life sciences Flaviviruses business.industry Organisms Zika Virus Cell Biology biology.organism_classification medicine.disease Rats Animal Models of Infection 030104 developmental biology Mixtures Animal Studies business 030217 neurology & neurosurgery |
| Zdroj: | PLoS ONE PLoS ONE, Vol 14, Iss 6, p e0218539 (2019) |
| ISSN: | 1932-6203 |
| Popis: | Zika virus (ZIKV) is a mosquito-borne flavivirus associated with microcephaly and other neurological disorders in infants born to infected mothers. Despite being declared an international emergency by the World Health Organization, very little is known about the mechanisms of ZIKV pathogenesis or the long-term consequences of maternal ZIKV infection in the affected offspring, largely due to the lack of appropriate rodent models. To address this issue, our lab has developed a working model of prenatal ZIKV infection in rats. In this study, we infected immune competent pregnant female rats with 105-107 PFU of ZIKV (PRVABC59, Puerto Rico/Human/Dec 2015) in order to examine its pathogenesis in the dams and pups. We examined the febrile response and sickness behavior in the dams, in addition to neonatal mortality, microglia morphology, cortical organization, apoptosis, and brain region-specific volumes in the offspring. Here, we demonstrate that pregnant and non-pregnant female rats have a distinct febrile response to ZIKV infection. Moreover, prenatal ZIKV infection increased cell death and reduced tissue volume in the hippocampus and cortex in the neonatal offspring. For the first time, we demonstrate the efficacy and validity of an immunocompetent rat model for maternal ZIKV infection that results in significant brain malformations in the neonatal offspring. |
| Databáze: | OpenAIRE |
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