Shedding light on cashmere goat hair follicle biology: from morphology analyses to transcriptomic landascape
| Autor: | Katia Cappelli, Stefano Capomaccio, Francesca Mercati, Samanta Mecocci, Irene Pazzaglia, Cristina Nocelli, Marco Antonini, Carlo Renieri, Luisa Pascucci |
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| Přispěvatelé: | Nocelli, C., Cappelli, K., Capomaccio, S., Pascucci, L., Mercati, F., Pazzaglia, I., Mecocci, S., Antonini, M., Renieri, C. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
lcsh:QH426-470
lcsh:Biotechnology Keratin 4 Real-Time Polymerase Chain Reaction Transcriptome 03 medical and health sciences Hair cycle lcsh:TP248.13-248.65 Gene expression Genetics medicine Cashmere goat Animals 030304 developmental biology Differentially expressed genes Hair follicle cycle RNAseq 0303 health sciences biology integumentary system Sequence Analysis RNA Gene Expression Profiling Goats 0402 animal and dairy science 04 agricultural and veterinary sciences Hair follicle 040201 dairy & animal science Cell biology Gene expression profiling body regions lcsh:Genetics medicine.anatomical_structure biology.protein Stem cell Hair Follicle Biotechnology Research Article |
| Zdroj: | BMC Genomics BMC Genomics, Vol 21, Iss 1, Pp 1-12 (2020) |
| ISSN: | 1471-2164 |
| Popis: | Background Cashmere goat is known for its precious undercoat. Being photoperiod-dictated, cashmere growth has been studied focusing mainly on hair follicle cycle phases (anagen, catagen and telogen). An accurate molecular knowledge of the goat hair follicle cycle, disentangling gene expression changes during phases and recognizing timing boundaries, could be useful to improve cashmere goat management and ultimately cashmere production. Results To better describe goat’s hair follicle transcriptome we applied RNA-sequencing to isolated hair follicles from five Italian cashmere goats, during the anagen and catagen phase, identifying total of 214 differentially expressed genes (DEGs): 97 were up-regulated while 117 were down-regulated in catagen with respect to anagen. Gene Ontology and pathway analysis were performed. We detected 144 significant pathways spanning from estrogen, pluripotency of stem cells, thermogenesis and fatty acid metabolism that were strongly expressed during the hair follicle phases analysed. Finally, we validated promising DEGs by RT-qPCR in the same set of samples as well as in hair follicles and entire skin biopsies of another cashmere goats cohort accounting for early anagen, anagen, early catagen, and catagen phases. Conclusions As in the isolated hair follicles, some target genes were homogenously modulated during the four hair follicle phases. Ceruloplasmin (CP) and Keratin 4 (K4), confirmed their clear cut expression between growing and resting phase. In fact, K4 was almost absent in catagen phases while CP was barely expressed in anagen phases. In particular, the strong expression of K4 in early anagen makes it an eligible marker to track the beginning of a new hair cycle, and therefore defining the optimum time for cashmere harvesting. |
| Databáze: | OpenAIRE |
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