Purification of recombinant hepatitis delta antigen expressed in E. coli cells
| Autor: | U. Barbieri, S. Osborne, Fabrizio Bonelli, M. Borla, Francis Poisson, Raffaele A. Calogero |
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| Rok vydání: | 1993 |
| Předmět: |
Recombinant Fusion Proteins
Biophysics Biology medicine.disease_cause Biochemistry Virus law.invention Antigen Structural Biology law Genetics medicine Escherichia coli Bovine serum albumin Molecular Biology Antigens Viral Purification Hepatitis Hepatitis delta antigen Cell Biology medicine.disease biology.organism_classification beta-Galactosidase Enterobacteriaceae Fusion protein Virology Molecular biology Recombinant DNA biology.protein RNA Viral Hepatitis Delta Virus |
| Zdroj: | FEBS letters. 318(3) |
| ISSN: | 0014-5793 |
| Popis: | Recombinant DNA technology enables the massive production of recombinant hepatitis delta antigen (recHDAg) retaining immunological properties and transport functions. However, purification procedures of the recombinant delta antigen have, to date, not been described in the literature. We present a purification procedure allowing one to obtain highly purified recHDAg from bacterial cells expressing the hepatitis delta antigen. |
| Databáze: | OpenAIRE |
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