In vitro kinetic study of growth and mineralization of osteoblast-like cells (Saos-2) on titanium surface coated with a RGD functionalized bisphosphonate

Autor: Daniel Chappard, David Portet, M.F. Moreau, Hélène Libouban, Johanne Beuvelot, Michel Félix Baslé, Grégory Lecollinet
Přispěvatelé: Groupe d'Études Remodelage Osseux et bioMatériaux (GEROM), Université d'Angers (UA), Laboratoires Goëmar S.A.S.
Jazyk: angličtina
Rok vydání: 2009
Předmět:
bisphosphonate
Materials science
Time Factors
Surface Properties
medicine.medical_treatment
[SDV]Life Sciences [q-bio]
Biomedical Engineering
chemistry.chemical_element
Nanotechnology
Biocompatible Materials
02 engineering and technology
In Vitro Techniques
Mineralization (biology)
Osseointegration
Cell Line
Biomaterials
03 medical and health sciences
Spectroscopy
Fourier Transform Infrared
medicine
Cell Adhesion
Image Processing
Computer-Assisted
Humans
titanium
Cell adhesion
030304 developmental biology
0303 health sciences
Osteoblasts
Diphosphonates
Osteoblast
surface treatment
Bisphosphonate
021001 nanoscience & nanotechnology
In vitro
adhesion molecule
Kinetics
medicine.anatomical_structure
chemistry
Biophysics
osteoblast
Titanium surface
Adsorption
0210 nano-technology
Peptides
Oligopeptides
Titanium
Zdroj: Journal of Biomedical Materials Reserach Part B Applied Biomaterials
Journal of Biomedical Materials Reserach Part B Applied Biomaterials, Wiley, 2009, 90B (2), pp.873-881. ⟨10.1002/jbm.b.31357⟩
ISSN: 1552-4981
DOI: 10.1002/jbm.b.31357⟩
Popis: International audience; Osteoconduction and osseointegration are the critical stages for implantation success. Peptides containing RGD (Arg-Gly-Asp) adhesive sequence are known to promote cell adhesion and consequently to favor osseointegration of medical devices. In this study, RGD peptides were coupled to a bisphosphonate used as an anchor system and chemically adsorbed on polished titanium discs. Two different concentrations, 10−10 mol/L (RGD 10−10) and 10−4 mol/L (RGD 10−4) were compared to non coated discs (RGD 0). Adhesion, spreading, and mineralization of osteoblast-like cells (Saos-2) were assessed. Mineralization kinetic was done at 3, 6, 10, 14, and 18 days of culture; the extent of mineral deposits was quantified by image analysis. Histogram repartitions of nuclear area, characterizing cell spreading, showed a shift to higher values in cells cultured on RGD coated titanium disks. Mineralization started at day 3 in the three groups, but had a faster development in the RGD 10−10 group from day 6 to day 18 compared to RGD 0 and RGD 10−4. At day 18, the percentage of mineralized area was significantly higher for RGD 10−10 compared to RGD 0 (p < 0.05). In the present study, this new method was found suitable to anchor RGD containing species on titanium: this favored adhesion and spreading of osteoblast-like cells and mineralization compared to noncoated titanium.
Databáze: OpenAIRE
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