IgE binding to linear epitopes of Ara h 2 in peanut allergic preschool children undergoing oral Immunotherapy

Autor:	A. Wesley Burks, Dominik Reinhold, Matthew Germinaro, Spodra Eglite, Caitlin M.G. McNulty, Werner Braun, Michael D. Kulis, Surendra S. Negi, Jeffery M. Chambliss, Xueni Chen, Stephen C. Dreskin, Brian P. Vickery
Rok vydání:	2019
Předmět:	Male Arachis Oral immunotherapy medicine.medical_treatment Immunology Peanut allergy Administration Oral Immunoglobulin E Peptide Mapping Ige binding Article Epitope Epitopes 03 medical and health sciences 0302 clinical medicine Food allergy medicine Animals Humans Immunology and Allergy Peanut Hypersensitivity 030212 general & internal medicine biology business.industry fungi food and beverages Immunotherapy Allergens Antigens Plant medicine.disease Tolerance induction 030228 respiratory system Desensitization Immunologic Child Preschool Pediatrics Perinatology and Child Health biology.protein Female business 2S Albumins Plant Protein Binding
Zdroj:	Pediatr Allergy Immunol
ISSN:	1399-3038 0905-6157
DOI:	10.1111/pai.13117
Popis:	BACKGROUND: For patients with peanut allergy, there are currently no methods to predict who will develop sustained unresponsiveness (SU) after oral immunotherapy (OIT). OBJECTIVE: Assess IgE binding to peanut (PN), Ara h 2 and to specific linear epitopes of Ara h 2 as predictors of the important clinical parameters: eliciting dose threshold and attainment of SU following OIT. METHODS: Samples and clinical data were collected from children undergoing OIT. PN- and Ara h 2-sIgE were quantified by ImmunoCap(®). IgE binding to linear peptides of Ara h 2 and Ara h 6 was measured with peptide microarrays. RESULTS: Lower values of PN-sIgE correlated with eliciting dose (P = 0.001) and with a higher likelihood of achieving SU (P < 0.0001) but these relationships were lost at higher values for PN-sIgE (≥ 14 kIU for eliciting dose and ≥ 35 kIU/L for SU). In subjects with PN-sIgE ≥ 14kIU/L, binding of IgE to epitopes 5 & 6 of Ara h 2 was associated with a lower eliciting dose at baseline challenge (P < 0.001; Pc < 0.02). In subjects with PN-sIgE ≥ 35kIU/L, a combined model of IgE binding to epitopes 1, 5 and 6 with PN-sIgE was highly predictive of attainment of SU (AUC of 0.86; P = 0.0067). CONCLUSION: In young patients with peanut allergy, measurement of PN-sIgE and IgE binding to specific linear epitopes of Ara h 2 in baseline samples may allow stratification of patients regarding sensitivity to challenge and outcome of OIT.
Databáze:	OpenAIRE
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