Detection of epitopes on follicle-stimulating hormone and FSH-antiserum-induced suppression of bioactivity of follicle-stimulating hormone and luteinizing hormone
| Autor: | R.H. Meloen, J.F. Flinterman, Wouter Cornelis Puijk, W.M.M. Schaaper, J. W. Slootstra, D. Kuperus, W. E. Westhoff, H.B. Oonk |
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| Rok vydání: | 1996 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Molecular Sequence Data Immunology Gonadotropin-releasing hormone Cross Reactions Biology Gonadotropic cell Antibodies Epitope Epitopes Follicle-stimulating hormone Internal medicine medicine Animals Humans Immunology and Allergy Amino Acid Sequence Rats Wistar Testosterone Immunosuppression Therapy Antiserum Sertoli Cells Sheep Sequence Homology Amino Acid Obstetrics and Gynecology Luteinizing Hormone Rats Contraception Endocrinology Reproductive Medicine Cattle Follicle Stimulating Hormone Luteinizing hormone Epitope Mapping hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Journal of Reproductive Immunology. 30:133-149 |
| ISSN: | 0165-0378 |
| DOI: | 10.1016/0165-0378(96)00955-2 |
| Popis: | There are currently two major approaches to hormonal male contraception. One relies on testosterone (analogs) either alone or in combination with gonadotropin releasing hormone (GnRH) (analogs or immunizations), the other on immunizations against follicle-stimulating hormone (FSH). Theoretically, the latter method will suppress spermatogenesis whilst not interfering with libido. An absolute requirement is, however, that an anti-FSH vaccine does not induce anti-luteinizing hormone (LH) antibodies (LH being responsible for the induction of testosterone which is necessary-to maintain libido). In this report we show that when whole FSH is used for vaccination, in most cases in addition to biological activity against FSH, anti-LH activity is also induced. By systematic analysis of the antisera raised with FSH using systematic epitope scanning (PEPSCAN) we found differences between the FSH-specific and FSH-nonspecific sera. Only the FSH-specific antiserum contained antibodies that recognized amino acid sequence 37–55 on the β-subunit in a linear manner. Because antibodies against this epitope have not been found in the cross-reactive sera this epitope forms a prime candidate for an anti-FSH contraceptive vaccine |
| Databáze: | OpenAIRE |
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