Enhanced production of plasmid DNA by engineered Escherichia coli strains
| Autor: | Eugenio Meza Mora, Alvaro R. Lara, Tania E. Pablos, René Soto, Guillermo Gosset, Octavio T. Ramírez, Sylvie Le Borgne |
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| Rok vydání: | 2011 |
| Předmět: |
Specific growth
Strain (chemistry) GalP Mutant Bioengineering General Medicine DNA Biology Acetates medicine.disease_cause Applied Microbiology and Biotechnology Molecular biology Microbiology Plasmid dna Mutation biology.protein medicine Escherichia coli Overflow metabolism Pyruvate kinase Biotechnology Plasmids |
| Zdroj: | Journal of biotechnology. 158(4) |
| ISSN: | 1873-4863 |
| Popis: | Escherichia coli strains VH33 (PTS − GalP + strain displaying a strongly reduced overflow metabolism) and VH34 (additionally lacking the pyruvate kinase A) were evaluated for the production of a plasmid DNA (pDNA) vaccine. The parent (W3110) and mutant strains were cultured using 10 g of glucose/L. While the specific growth rates of the three strains were similar, they presented differences in the accumulation of acetate. W3110 accumulated up to 4 g/L of acetate, VH33 produced 1.4 g/L, and VH34 only 0.78 g/L. VH33 and VH34 produced 76% and 300% more pDNA than W3110. Moreover, VH34 demanded 33% less oxygen than VH33 and W3110, which can be advantageous for large-scale applications. |
| Databáze: | OpenAIRE |
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