Structured PDMS Chambers for Enhanced Human Neuronal Cell Activity on MEA Platforms

Autor:	Jarno E. Mikkonen, Heli Skottman, Susanna Narkilahti, Pasi Kallio, Joose Kreutzer, Laura Ylä-Outinen, Tiina Kaarela, Paula Kärnä
Přispěvatelé:	Tampere University, Institute of Biomedical Technology, Department of Automation Science and Engineering, Integrated Technologies for Tissue Engineering Research (ITTE)
Rok vydání:	2012
Předmět:	Materials science Cell seeding Coating materials 3112 Neurosciences technology industry and agriculture Biophysics Bioengineering Nanotechnology 222 Other engineering and technologies macromolecular substances Multielectrode array Cell activity Chamber design Biotechnology Biomedical engineering
Zdroj:	Journal of Bionic Engineering. 9:1-10
ISSN:	2543-2141 1672-6529
DOI:	10.1016/s1672-6529(11)60091-7
Popis:	Structured poly(dimethylsiloxane) (PDMS) chambers were designed and fabricated to enhance the signaling of human Embryonic Stem Cell (hESC) – derived neuronal networks on Microelectrode Array (MEA) platforms. The structured PDMS chambers enable cell seeding on restricted areas and thus, reduce the amount of needed coating materials and cells. In addition, the neuronal cells formed spontaneously active networks faster in the structured PDMS chambers than that in control chambers. In the PDMS chambers, the neuronal networks were more active and able to develop their signaling into organized signal trains faster than control cultures. The PDMS chamber design enables much more repeatable analysis and rapid growth of functional neuronal network in vitro. Moreover, due to its easy and cheap fabrication process, new configurations can be easily fabricated based on investigator requirements.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9cda33bf5604ac4de28b2015927018af https://doi.org/10.1016/s1672-6529(11)60091-7 Zobrazit plný text záznamu Full Text from ScienceDirect Full text from SpringerLink