The Cellular Origins of the Outer Retinal Bands in Optical Coherence Tomography Images
| Autor: | Ravi S. Jonnal, Donald T. Miller, Sang-Hyuck Lee, John S. Werner, Robert J. Zawadzki, Omer P. Kocaoglu |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
genetic structures
Ophthalmological Eye Ophthalmology & Optometry Medical and Health Sciences Retina Basement Membrane adaptive optics Cellular and Molecular Neuroscience chemistry.chemical_compound Optics Optical coherence tomography Clinical Research Models Perifovea medicine Humans Retinal Photoreceptor Cell Inner Segment External limiting membrane Eye Disease and Disorders of Vision Tomography Physics optical coherence tomography medicine.diagnostic_test business.industry photoreceptor morphology Anatomic Neurosciences Retinal Articles Biological Sciences Retinal Photoreceptor Cell Outer Segment Ellipsoid Sensory Systems Diagnostic Techniques Ophthalmology medicine.anatomical_structure chemistry Optical Coherence Retinal Cone Photoreceptor Cells Biomedical Imaging nomenclature sense organs business morphometry Coherence (physics) |
| Zdroj: | Investigative ophthalmology & visual science, vol 55, iss 12 Jonnal, RS; Kocaoglu, OP; Zawadzki, RJ; Lee, SH; Werner, JS; & Miller, DT. (2014). The cellular origins of the outer retinal bands in optical coherence tomography images. Investigative Ophthalmology and Visual Science, 55(12), 7904-7918. doi: 10.1167/iovs.14-14907. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/116541j1 |
| DOI: | 10.1167/iovs.14-14907. |
| Popis: | Author(s): Jonnal, Ravi S; Kocaoglu, Omer P; Zawadzki, Robert J; Lee, Sang-Hyuck; Werner, John S; Miller, Donald T | Abstract: PurposeTo test the recently proposed hypothesis that the second outer retinal band, observed in clinical OCT images, originates from the inner segment ellipsoid, by measuring: (1) the thickness of this band within single cone photoreceptors, and (2) its respective distance from the putative external limiting membrane (band 1) and cone outer segment tips (band 3).MethodsAdaptive optics-optical coherence tomography images were acquired from four subjects without known retinal disease. Images were obtained at foveal (2°) and perifoveal (5°) locations. Cone photoreceptors (n = 9593) were identified and segmented in three dimensions using custom software. Features corresponding to bands 1, 2, and 3 were automatically identified. The thickness of band 2 was assessed in each cell by fitting the longitudinal reflectance profile of the band with a Gaussian function. Distances between bands 1 and 2, and between 2 and 3, respectively, were also measured in each cell. Two independent calibration techniques were employed to determine the depth scale (physical length per pixel) of the imaging system.ResultsWhen resolved within single cells, the thickness of band 2 is a factor of three to four times narrower than in corresponding clinical OCT images. The distribution of band 2 thickness across subjects and eccentricities had a modal value of 4.7 μm, with 48% of the cones falling between 4.1 and 5.2 μm. No significant differences were found between cells in the fovea and perifovea. The distance separating bands 1 and 2 was found to be larger than the distance between bands 2 and 3, across subjects and eccentricities, with a significantly larger difference at 5° than 2°.ConclusionsOn the basis of these findings, we suggest that ascription of the outer retinal band 2 to the inner segment ellipsoid is unjustified, because the ellipsoid is both too thick and proximally located to produce the band. |
| Databáze: | OpenAIRE |
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