Methylmercury determination in freshwater biota and sediments: Static headspace GC-MS compared to direct mercury analyzer
| Autor: | Alfredo Schiavon, Claudio Roscioli, Laura Marziali, Lucia Valsecchi |
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| Rok vydání: | 2021 |
| Předmět: |
Spectrum analyzer
Science Clinical Biochemistry chemistry.chemical_element Biota Organometallic compounds Method Article Mercury (element) Sediments Medical Laboratory Technology chemistry.chemical_compound chemistry Environmental chemistry Benthic invertebrates Methylmercury determination Gas chromatography–mass spectrometry Methylmercury |
| Zdroj: | MethodsX MethodsX, Vol 8, Iss, Pp 101581-(2021) |
| ISSN: | 2215-0161 |
| Popis: | We developed and compared two analytical methods for determination of MeHg in freshwater biota and sediments, by: I) simplified static headspace GC-MS using internal standard (IS) isotope dilution quantification, after microwave acid digestion and aqueous phase NaBEt4 ethylation; II) Automated Mercury Analyzer, after double toluene extraction followed by back-extraction with L-cystein. The performance was evaluated by analysis of certified reference materials. For biota, mean recovery was 100 ± 2% and relative standard deviation (RSD) ≤ 6.8% for method I, and mean recovery was 98 ± 7% and RSD ≤13% for method II. For sediments, recovery of 94.5% and RSD of 8.8% were obtained with method I, and recovery of 90.3% and RSD of 9.4% with method II. Limits of detection (LOD) were 0.7 µg kg−1 and 6 µg kg−1, respectively. Both techniques were tested for MeHg analysis in freshwater invertebrates, fish and sediments, covering a large range of MeHg values (1.9–670 µg kg−1 d.w.). • Both protocols proved to be suitable for MeHg analysis in complex environmental matrices, even if, for method II, interferences in the extraction phase and limited sensitivity may hinder sediment analysis. • Passing-Bablock regression revealed a slight disproportion between methods, with line slope = 1.058 (95% CI ranging from 1.001 to 1.090). Graphical abstract Image, graphical abstract |
| Databáze: | OpenAIRE |
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