First Human Cell Experiments With FLASH Carbon Ions

Autor:	MUTSUMI TASHIRO, YUKARI YOSHIDA, TAKAHIRO OIKE, MASAO NAKAO, KEN YUSA, YUKA HIROTA, TATSUYA OHNO
Rok vydání:	2022
Předmět:	Ions Cancer Research Oncology Humans Heavy Ion Radiotherapy General Medicine Fibroblasts Carbon
Zdroj:	Anticancer Research. 42:2469-2477
ISSN:	1791-7530 0250-7005
Popis:	This study aimed to establish a setup for ultra-high-dose-rate (FLASH) carbon-ion irradiation, and to conduct the first human cell experiments using FLASH carbon ions.A system for FLASH carbon-ion irradiation (1-3 Gy at 13 or 50 keV/μm) was developed. The growth and senescence of HFL1 lung fibroblasts were assessed by crystal violet staining assays and senescence-associated β-galactosidase staining, respectively. Survival of HSGc-C5 cancer cells was assessed by clonogenic assays.The dose rates of carbon ions ranged from 96-195 Gy/s, meeting the definition of FLASH. With both 13 and 50 keV/μm beams, no FLASH sparing effect was observed on the growth suppression and senescence of HFL1 cells, nor on the survival of HSGc-C5 cells.We successfully conducted the first human cell experiments with FLASH carbon ions. No FLASH effect was observed under the conditions examined.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9cd200903b9b92be19d62c48f6f4da45 https://doi.org/10.21873/anticanres.15725 Zobrazit plný text záznamu