Effect of essential fatty acid deficiency on G-proteins, cAMP-dependent protein kinase activity and mucin secretion in the rat submandibular salivary glands
| Autor: | Ibrahim Y. Ibrahim, Selim M. Abdel-Hakim, Bassima S. Alam, Syed Q. Alam |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
medicine.medical_specialty G protein Submandibular Gland Biology CAMP-dependent protein kinase activity Rats Sprague-Dawley Essential fatty acid GTP-Binding Proteins Internal medicine Receptors Adrenergic beta Cyclic AMP medicine Animals Secretion Salivary Proteins and Peptides Saliva Protein kinase A Receptor chemistry.chemical_classification Fatty Acids Essential Body Weight Mucins Cell Biology Cyclic AMP-Dependent Protein Kinases Dietary Fats Rats Endocrinology chemistry Signal transduction Secretory Rate Glycoprotein Adenylyl Cyclases Signal Transduction |
| Zdroj: | Cellular Signalling. 7:773-781 |
| ISSN: | 0898-6568 |
| DOI: | 10.1016/0898-6568(95)02005-5 |
| Popis: | Studies were conducted to determine whether beta-adrenergic cell signalling is altered in submandibular salivary glands (SMSG) is essential fatty acid (EFA) deficiency. Three groups of rats were fed diets which were deficient in EFA (EFAD), marginally deficient in EFA (MEFAD) or contained sufficient amount of EFA (Control). Rats were killed after 20 wk on diets, SMSG were dissected out and cyclic AMP-dependent protein kinase (PKA) activity was measured. The specific enzyme activities were higher in the homogenates and supernatant fractions of the gland from EFAD and MEFAD rats compared with the controls. The relative levels of guanine nucleotide-binding regulatory proteins (Gs and Gi) were also measured in the SMSG membranes of rats fed the 3 diets. The levels of Gs were significantly higher in the EFAD and MEFAD groups than in the controls. No significant differences were observed in the secretion of trichloroacetic acid-phosphotungstic acid (TCA-PTA) precipitable glycoproteins from the SMSG slices among the 3 dietary groups. |
| Databáze: | OpenAIRE |
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