Physico-chemical characterization and antibacterial activity of inclusion complexes of Hyptis martiusii Benth essential oil in β-cyclodextrin
| Autor: | Paula Santos Nunes, Fábia F. Campina, Mairim Russo Serafini, Grace Anne Azevedo Dória, Francielly de Oliveira Araujo, Henrique Douglas Melo Coutinho, Irwin Rose Alencar de Menezes, Maria do Socorro Costa, Antonia Thassya Lucas dos Santos, Alessandra Silva Rabelo, Thiago Sampaio de Freitas, Ana Raquel Pereira da Silva, Tatianny de Araújo Andrade, Márcio R. V. Santos, Daniel P. Bezerra, Paula dos Passos Menezes, Adriano Antunes de Souza Araújo, Lucindo José Quintans-Júnior |
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| Rok vydání: | 2017 |
| Předmět: |
Staphylococcus aureus
Hyptis Drug Compounding Beta-Cyclodextrins 02 engineering and technology 01 natural sciences law.invention Differential scanning calorimetry X-Ray Diffraction law Gram-Negative Bacteria Spectroscopy Fourier Transform Infrared Oils Volatile Solubility Essential oil Pharmacology Cyclodextrins Calorimetry Differential Scanning biology Plant Extracts 010405 organic chemistry Chemistry beta-Cyclodextrins General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Anti-Bacterial Agents 0104 chemical sciences Thermogravimetry 0210 nano-technology Antibacterial activity Karl Fischer titration Nuclear chemistry |
| Zdroj: | Biomedicine & Pharmacotherapy. 89:201-207 |
| ISSN: | 0753-3322 |
| DOI: | 10.1016/j.biopha.2017.01.158 |
| Popis: | Cyclodextrins (CDs) have been used as important pharmaceutical excipients for improve the physicochemical properties of the drugs of low solubility as the essential oil of Hyptis martiusii. This oil is important therapeutically, but the low solubility and bioavailability compromises your use. Therein, the aim of this study was to obtain and to characterize physico-chemically the samples obtained by physical mixture (PM), paste complexation (PC) and slurry complexation (SC) of the essential oil Hyptis martiusii (EOHM) in β-CD, and to compare the antibacterial and modulatory-antibiotic activity of products obtained and oil free. The physicochemical characterization was performed by differential scanning calorimetry (DSC), thermogravimetry/derivative thermogravimetry (TG/DTG), scanning electron microscopy (SEM), X-ray diffraction (XRD) and Karl Fischer titration. Additionally, the antibacterial tests were performed by microdilution technique. Thus, it was observed that the PM method showed low complexing capacity, unlike PC and SC in which it was observed the formation of inclusion complexes. In addition, the second stage of the TG/DTG curves showed that SC was the best method inclusion with mass loss of 6.9% over the PC that was 6.0%. The XRD results corroborate with the results above suggesting the formation of new solid phase and the SEM photomicrographs showed the porous surface of the samples PC and SC. The essential oil alone demonstrated an antibacterial and modulatory effect against the S. aureus and the Gram negative strain, respectively. However, the β-CD and the inclusion complex did not demonstrate any biological activity in the performed antibacterial assays. |
| Databáze: | OpenAIRE |
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