Comparison of four serological assays for the diagnosis of Chlamydia trachomatis in subfertile women
| Autor: | Marleen Temmerman, Petra De Sutter, Claude Mambo Muvunyi, Lieve Van Renterghem, Laurens Claeys, Elizaveta Padalko, Geert Claeys, Tineka De Sutter |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Infertility
medicine.medical_specialty subfertility serology Fluorescent Antibody Technique Chlamydia antibodies Chlamydia trachomatis Enzyme-Linked Immunosorbent Assay TUBAL FACTOR SUBFERTILITY medicine.disease_cause Microbiology Sensitivity and Specificity Immunoglobulin G Serology antibody Virology ANTIBODY-TITERS INFECTION Medicine and Health Sciences Medicine Humans METAANALYSIS DAMAGE Gynecology biology IGG business.industry Clinical Laboratory Techniques Antibody titer INFERTILE WOMEN General Medicine Gold standard (test) Chlamydia Infections medicine.disease MICROIMMUNOFLUORESCENCE ASSAY Antibodies Bacterial female genital diseases and pregnancy complications Infectious Diseases TESTS biology.protein IMMUNOGLOBULIN-G Parasitology Female Antibody business |
| Zdroj: | JOURNAL OF INFECTION IN DEVELOPING COUNTRIES |
| ISSN: | 1972-2680 |
| Popis: | Introduction: Chlamydia antibody testing (CAT) in serum has been introduced as a screening method in the infertility workup. We evaluated the test characteristics of two ELISA tests compared to micro-immunofluorescence tests (MIFs). MIFs are considered the gold standard in the C. trachomatis IgG antibodies detection. We also compared the accuracy of all CAT tests in predicting tubal subfertility, using laparoscopy as a reference. Methodology: Four commercial serological methods were used to analyse 101 serum samples for the presence of C. trachomatis IgG antibodies from patients at the Infertility Clinic of Ghent University Hospital. The diagnostic utility for prediction of tubal infertility of serological methods was evaluated based on patients' medical records. Results: A comparison of the serological assays showed little difference in the major performance characteristics: the sensitivities of all MIFs and ELISAs were 100% for all assays (except the ELISA Vircell, with a sensitivity of 90%), and the specificities ranged from 92% for MIF Ani Labsystems to 98% for the MIF Focus and ELISA Vircell. As compared to laparoscopy data, CAT positivity in subfertile women with tubal damage (n=40) did not significantly differ from that of subfertile women without tubal damage (n=61): Positive predictive values (PPV) of CAT ranged from 53% to 60% and negative predictive values (NPV) ranged from 62% to 64%. Conclusion: evaluated ELISAs are comparable to MIFs in the detection of C. trachomatis IgG antibodies and should be preferred for large serological studies, especially in resource poor settings. |
| Databáze: | OpenAIRE |
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