Nanoparticle-Mediated Cell Capture Enables Rapid Endothelialization of a Novel Bare Metal Stent
| Autor: | Rajiv Gulati, Peter J. Psaltis, Adriana Harbuzariu, Susheil Uthamaraj, David R. Holmes, Ota Hlinomaz, Robert D. Simari, Brant Newman, Gurpreet S. Sandhu, Brandon J. Tefft, J. Jonathan Harburn, Tyra A. Witt, Dan Dragomir-Daescu |
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| Rok vydání: | 2018 |
| Předmět: |
Bare-metal stent
Materials science Swine Cell Biomedical Engineering Nanoparticle Bioengineering 02 engineering and technology 030204 cardiovascular system & hematology Biochemistry Biomaterials 03 medical and health sciences 0302 clinical medicine medicine Animals cardiovascular diseases Stent thrombosis Endothelial Cells Original Articles Stainless Steel equipment and supplies 021001 nanoscience & nanotechnology Cell targeting Phenotype surgical procedures operative medicine.anatomical_structure Metals Nanoparticles Female Stents 0210 nano-technology Biomedical engineering |
| Zdroj: | Tissue Engineering Part A. 24:1157-1166 |
| ISSN: | 1937-335X 1937-3341 |
| Popis: | Incomplete endothelialization of intracoronary stents has been associated with stent thrombosis and recurrent symptoms, whereas prolonged use of dual antiplatelet therapy increases bleeding-related adverse events. Facilitated endothelialization has the potential to improve clinical outcomes in patients who are unable to tolerate dual antiplatelet therapy. The objective of this study was to demonstrate the feasibility of magnetic cell capture to rapidly endothelialize intracoronary stents in a large animal model. A novel stent was developed from a magnetizable duplex stainless steel (2205 SS). Polylactic-co-glycolic acid and magnetite (Fe(3)O(4)) were used to synthesize biodegradable superparamagnetic iron oxide nanoparticles, and these were used to label autologous blood outgrowth endothelial cells. Magnetic 2205 SS and nonmagnetic 316L SS control stents were implanted in the coronary arteries of pigs (n = 11), followed by intracoronary delivery of magnetically labeled cells to 2205 SS stents. In this study, we show extensive endothelialization of magnetic 2205 SS stents (median 98.4% cell coverage) within 3 days, whereas the control 316L SS stents exhibited significantly less coverage (median 48.9% cell coverage, p |
| Databáze: | OpenAIRE |
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