Organoids and organs-on-chips: Insights into human gut-microbe interactions
| Autor: | Jens Puschhof, Cayetano Pleguezuelos-Manzano, Hans Clevers |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Culture model
Microfluidics Computational biology Gut flora Animal Testing Alternatives Microbiology Organ-on-a-chip 03 medical and health sciences Human health Mice 0302 clinical medicine Human gut Organoids/microbiology Models Virology Microchip Analytical Procedures Organoid Animals Humans Microbiome Microfluidics/methods Intestinal Mucosa Precision Medicine 030304 developmental biology 0303 health sciences biology Host Microbial Interactions Animal Precision Medicine/methods biology.organism_classification Microchip Analytical Procedures/methods Coculture Techniques Gastrointestinal Microbiome Organoids Metagenomics Models Animal Microbial Interactions Parasitology Coculture Techniques/methods 030217 neurology & neurosurgery |
| Zdroj: | Cell Host & Microbe |
| ISSN: | 1934-6069 |
| Popis: | Summary The important and diverse roles of the gut microbiota in human health and disease are increasingly recognized. The difficulty of inferring causation from metagenomic microbiome sequencing studies and from mouse-human interspecies differences has prompted the development of sophisticated in vitro models of human gut-microbe interactions. Here, we review recent advances in the co-culture of microbes with intestinal and colonic epithelia, comparing the rapidly developing fields of organoids and organs-on-chips with other standard models. We describe how specific individual processes by which microbes and epithelia interact can be recapitulated in vitro. Using examples of bacterial, viral, and parasitic infections, we highlight the advantages of each culture model and discuss current trends and future possibilities to build more complex co-cultures. |
| Databáze: | OpenAIRE |
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