Noninvasive Subcellular Imaging Using Atomic Force Acoustic Microscopy (AFAM)
| Autor: | Li Su, Jing Wang, Wenjie Deng, Xiaoqing Li, Ang Lu, Mingyue Ding |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Image fusion algorithm
Materials science Acoustics Phase (waves) Microscopy Acoustic Atomic force acoustic microscopy 02 engineering and technology 010402 general chemistry Microscopy Atomic Force 01 natural sciences Signal Article subcellular structural image atlas Imaging Three-Dimensional Cell Line Tumor High spatial resolution Humans lcsh:QH301-705.5 image fusion algorithm General Medicine 021001 nanoscience & nanotechnology atomic force acoustic microscopy 0104 chemical sciences lcsh:Biology (General) Transmission (telecommunications) parameters optimization 0210 nano-technology Algorithms Subcellular Fractions |
| Zdroj: | Cells Volume 8 Issue 4 Cells, Vol 8, Iss 4, p 314 (2019) |
| ISSN: | 2073-4409 |
| Popis: | We report an imaging approach applying the atomic force acoustic microscopy (AFAM), which has unique potential for nondestructive imaging of cell internal structures. To obtain high spatial resolution images, we optimized the significant imaging parameters, including scanning speeds, feedback configurations and acoustic frequencies of an AFAM system, to increase the amplitude of the acoustic signal and to stabilize the morphological signals. We also combined the acoustic amplitude and phase signals, and generated pseudo-color figures for better illustration of subcellular features such as pseudopodia, membranes and nucleus-like. The subcellular structural image atlas can describe nanoscale details of multiple samples and provide clearer images of the subcellular features compared to other conventional techniques. This study builds a strong basis of transmission AFAM for cell imaging, which can help researchers to clarify the cell structures in diverse biological fields and push the understanding of biology evolution to a new stage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |