Thioredoxin Blood Level Increases After Severe Burn Injury
| Autor: | Bita Sahaf, Junji Yodoi, Avni Abdiu, Arne Holmgren, Hajime Nakamura, Anders Rosén |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Blood Platelets Male Blood level animal structures Antioxidant Adolescent Physiology medicine.medical_treatment Blotting Western Clinical Biochemistry Enzyme-Linked Immunosorbent Assay macromolecular substances Pharmacology Biochemistry Antioxidants Thioredoxins medicine Humans Severe burn Microscopy Immunoelectron Molecular Biology Aged General Environmental Science business.industry Case-control study Cell Biology Middle Aged Blot Case-Control Studies Immunology General Earth and Planetary Sciences Female Thioredoxin Burns business |
| Zdroj: | Antioxidants & Redox Signaling. 2:707-716 |
| ISSN: | 1557-7716 1523-0864 |
| Popis: | We have investigated the thioredoxin (TRX) levels in severely burned patients and the possible origin of TRX, based on the recent understanding that TRX is a potent antioxidant with cytoprotective functions. Serum and plasma samples from burns patients and healthy blood donors were collected during the first 10 post-burn days and analyzed in a sandwich TRX enzyme-linked immunosorbent assay (ELISA). The TRX levels found were correlated to a panel of blood tests. The presence of TRX in platelets was investigated by immunoelectron microscopy and Western blotting. TRX serum levels of the severely burned patients showed a significant increase, with a mean serum TRX concentration on the day of injury of 76.5 +/- 19.5 ng/ml (mean +/- SD) and on post-burn day one 122.6 +/- 66.9 ng/ml, compared to control blood donor levels of 22.7 +/- 12.2 ng/ml (p = 0.0041 and 0.0117, respectively). A second peak of increase was found on post-burn days 7 to 9 with a four- to five-fold rise in concentration compared to controls. TRX elevation correlated well with increased platelet (p = 0.007) and leukocyte counts (p = 0.002). We also demonstrated by immunoelectron microscopy and Western blotting the presence of TRX in platelets. In conclusion, our demonstration of TRX release in burn injuries indicates that the TRX system is involved in a rapid antioxidant defense, coagulation processes, cell growth, and control of the extracellular peroxide tone intimately linked to cytoprotection and wound healing in burns. One of the cell types that delivers TRX promptly and efficiently into the blood may be the platelet. |
| Databáze: | OpenAIRE |
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