(Hyper)thermophilic enzymes : Production and purification

Autor: Pierpaolo Falcicchio, Sotirios Koutsopoulos, Servé W. M. Kengen, John van der Oost, Mark Levisson
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Protein Downstream Processing
Methods in Molecular Biology ISBN: 9781071607749
Protein Downstream Processing. Totowa: Humana Press
Methods in Molecular Biology ISBN: 9781627039765
Protein Downstream Processing. Humana Press Inc.
Popis: The discovery of thermophilic and hyperthermophilic microorganisms, thriving at environmental temperatures near or above 100 °C, has revolutionized our ideas about the upper temperature limit at which life can exist. The characterization of (hyper)thermostable proteins has broadened our understanding and presented new opportunities for solving one of the most challenging problems in biophysics: how are structural stability and biological function maintained at high temperatures where “normal” proteins undergo dramatic structural changes? In our laboratory, we have purified and studied many thermostable and hyperthermostable proteins in an attempt to determine the molecular basis of heat stability. Here, we present methods to express such proteins and enzymes in E. coli and provide a general protocol for overproduction and purification. The ability to produce enzymes that retain their stability and activity at elevated temperatures creates exciting opportunities for a wide range of biocatalytic applications.
Databáze: OpenAIRE