Biological applications of an LCoS-based programmable array microscope (PAM)

Autor:	Martin Thomas, Thomas M. Jovin, Guy M. Hagen, Andrew H. Hill, Cornelia Fritsch, Bernd Rieger, Bert van Geest, Donna J. Arndt-Jovin, Keith A. Lidke, Wouter Caarls
Rok vydání:	2007
Předmět:	FLIM Materials science Microscope Spatial light modulator emCCD Optical sectioning business.industry EGFR Inverted microscope quantum dots Laser fluorescence microscopy DRONPA SLM law.invention Liquid crystal on silicon Dronpa confocal law Temporal resolution erbB FRET Optoelectronics business
Zdroj:	Proceedings of SPIE Proceedings of SPIE, 2007 vol. 6441
ISSN:	0277-786X
DOI:	10.1117/12.710995
Popis:	We report on a new generation, commercial prototype of a programmable array optical sectioning fluorescence microscope (PAM) for rapid, light efficient 3D imaging of living specimens. The stand-alone module, including light source(s) and detector(s), features an innovative optical design and a ferroelectric liquid-crystal-on-silicon (LCoS) spatial light modulator (SLM) instead of the DMD used in the original PAM design. The LCoS PAM (developed in collaboration with Cairn Research, Ltd.) can be attached to a port of a(ny) unmodified fluorescence microscope. The prototype system currently operated at the Max Planck Institute incorporates a 6-position high-intensity LED illuminator, modulated laser and lamp light sources, and an Andor iXon emCCD camera. The module is mounted on an Olympus IX71 inverted microscope with 60-150X objectives with a Prior Scientific x,y, and z high resolution scanning stages. Further enhancements recently include: (i) point- and line-wise spectral resolution and (ii) lifetime imaging (FLIM) in the frequency domain. Multiphoton operation and other nonlinear techniques should be feasible. The capabilities of the PAM are illustrated by several examples demonstrating single molecule as well as lifetime imaging in live cells, and the unique capability to perform photoconversion with arbitrary patterns and high spatial resolution. Using quantum dot coupled ligands we show real-time binding and subsequent trafficking of individual ligand-growth factor receptor complexes on and in live cells with a temporal resolution and sensitivity exceeding those of conventional CLSM systems. The combined use of a blue laser and parallel LED or visible laser sources permits photoactivation and rapid kinetic analysis of cellular processes probed by photoswitchable visible fluorescent proteins such as DRONPA.
Databáze:	OpenAIRE
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