Radical scavenging and antibacterial activity of Arnebia benthamii methanol extract
| Autor: | Showkat Ahmad Ganie, Rabia Hamid, Bilal A. Zargar, Asima Jan, Sabeera Muzaffar, M. Afzal Zargar |
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| Rok vydání: | 2012 |
| Předmět: |
Antioxidant
DNA damage DPPH medicine.medical_treatment TBARS Free radicals Arnebia benthamii Thiobarbituric Acid Reactive Substances Antioxidants law.invention Lipid peroxidation chemistry.chemical_compound law medicine Escherichia coli Animals Humans chemistry.chemical_classification Medicine(all) Reactive oxygen species Traditional medicine Plant Extracts Methanol food and beverages General Medicine Free Radical Scavengers Boraginaceae Anti-Bacterial Agents Rats Plant Leaves chemistry Antibacterial activity Lipid Peroxidation Phytotherapy Reactive Oxygen Species Oxidation-Reduction |
| Zdroj: | Asian Pacific journal of tropical medicine. 5(10) |
| ISSN: | 2352-4146 |
| Popis: | ObjectiveTo evaluate in vitro antioxidant and antibacterial activity of methanolic extract of Arnebia benthamii (A. benthamii) whole plant.MethodsPlasmid damage was analyzed by agarose gell electrophoresis. Calf thymus DNA was monitored by TBARS formation. DPPH, reducing power and lipid peroxidation was evaluated by using standard procedures. Antibacterial assay was monitored by disc diffusion method.ResultsDPPH radical scavenging and hydroxyl radical scavenging potential of the plant revealed that the extract to be active radical scavenger. Reducing (Fe3+-Fe2+) power and lipid peroxidation inhibition efficiency (TBARS assay) of the extract was also evaluated and the extract showed promising activity in preventing lipid peroxidation and might prevent oxidative damages to biomolecules. The extract offered a significant protection against plasmid and calf thymus DNA damage induced by hydroxyl radicals. The extract was also evaluated on different bacterial strains and the maximum antibacterial activity was exhibited against Escherichia coli (E. coli) when compared with standard drug.ConclusionsThese findings demonstrate that the methanol extract of A. benthamii has excellent anti-oxidant activities and could be considered as a potential source of lead molecules for pharmaceutical industries. |
| Databáze: | OpenAIRE |
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