Salicylic Acid Mediated by the Oxidative Burst Is a Key Molecule in Local and Systemic Responses of Cotton Challenged by an Avirulent Race of Xanthomonas campestris pvmalvacearum
| Autor: | Michel Nicole, Jean-Paul Geiger, Aida Jalloul, Jean-Claude Baccou, Jean-François Daniel, E. Bresson, Komi Assigbetse, Jean-Luc Montillet, Christelle Martinez, Yves Baissac |
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| Rok vydání: | 2000 |
| Předmět: |
ACIDE SALICYLIQUE
Hypersensitive response COTYLEDON Physiology BACTERIE Plant Science Xanthomonas campestris MALADIE DES PLANTES chemistry.chemical_compound INFECTION Genetics MECANISME DE RESISTANCE COTON Amitrole Plant Diseases Respiratory Burst chemistry.chemical_classification Gossypium Reactive oxygen species Virulence biology Inoculation PEROXIDASE food and beverages Hydrogen Peroxide OXYGENE biology.organism_classification Molecular biology Respiratory burst Kinetics Peroxidases Biochemistry chemistry Catalase biology.protein ETUDE EXPERIMENTALE Reactive Oxygen Species Salicylic Acid Cotyledon Salicylic acid Signal Transduction Research Article Peroxidase |
| Zdroj: | Plant Physiology. 122:757-766 |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.122.3.757 |
| Popis: | We analyzed the production of reactive oxygen species, the accumulation of salicylic acid (SA), and peroxidase activity during the incompatible interaction between cotyledons of the cotton (Gossypium hirsutum) cv Reba B50/Xanthomonas campestris pvmalvacearum (Xcm) race 18. SA was detected in petioles of cotyledons 6 h after infection and 24 h post inoculation in cotyledons and untreated leaves. The first peak of SA occurred 3 h after generation of superoxide (O2 ·−), and was inhibited by infiltration of catalase. Peroxidase activity and accumulation of SA increased in petioles of cotyledons and leaves following H2O2 infiltration of cotyledons from 0.85 to 1 mm. Infiltration of 2 mm SA increased peroxidase activity in treated cotyledons and in the first leaves, but most of the infiltrated SA was rapidly conjugated within the cotyledons. When increasing concentrations of SA were infiltrated 2.5 h post inoculation at the beginning of the oxidative burst, the activity of the apoplastic cationic O2 ·−-generating peroxidase decreased in a dose-dependent manner. We have shown that during the cotton hypersensitive response to Xcm, H2O2 is required for local and systemic accumulation of SA, which may locally control the generation of O2 ·−. Detaching cotyledons at intervals after inoculation demonstrated that the signal leading to systemic accumulation of SA was emitted around 3 h post inoculation, and was associated with the oxidative burst. SA produced 6 h post infection at HR sites was not the primary mobile signal diffusing systemically from infected cotyledons. |
| Databáze: | OpenAIRE |
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