Vaccination against Taenia taeniaeformis infection in rats using a recombinant protein and preliminary analysis of the induced antibody response
| Autor: | Tsuyoshi Takami, Graham F. Mitchell, H. O. Bøgh, Marshall W. Lightowlers, Akira Ito, Masao Kamiya, Kazuo Onitake, Michael D. Rickard |
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| Rok vydání: | 1991 |
| Předmět: |
Male
Recombinant Fusion Proteins Antibodies Helminth Taenia taeniaeformis Antigen Animals Cloning Molecular Molecular Biology Glutathione Transferase Taeniasis Vaccines Synthetic Taenia biology Schistosoma japonicum Vaccination Oncosphere Rats Inbred Strains Helminth Proteins biology.organism_classification Molecular biology Fusion protein Rats Specific Pathogen-Free Organisms Glutathione S-transferase Antigens Helminth biology.protein Parasitology Antibody |
| Zdroj: | Molecular and Biochemical Parasitology. 44:43-49 |
| ISSN: | 0166-6851 |
| DOI: | 10.1016/0166-6851(91)90219-v |
| Popis: | Primary screening of a cDNA expression library of Taenia taeniaeformis oncospheres in λgt11 bacteriophage was carried out using rabbit anti- T. taeniaeformis oncosphere serum affinity-purified from disrupted oncosphere pellets. From approximately 1.6 × 10 5 plaques, 21 single clones that were positive with the affinity-purified antibodies were isolated. Sibling analysis revealed that 17 clones out of the 21 could be assigned to five different antigen families. Only family 1 was strongly recognized by a serum prepared in a rabbit against a partially purified host-protective oncosphere antigen fraction. The fragments of λDNA were inserted into a pGEX plasmid vector that encodes glutathione S- transferase (GST) of Schistosoma japonicum . Clones designated TtO-18, −49, 53 (family 1), 46 (family 2), 15 (family 3), 40 (family 4) and 66 (family 5) were established as subclones in pGEX-1 plasmid vectors which produced GST fusion proteins. All GST fusion proteins were soluble and recognized by anti-GST and anti-TtO sera. Three vaccination experiments with these fusion proteins using specific-pathogen-free Wistar rats revealed that all three fusion proteins of family 1 were exclusively effective against T. taeniaeformis oncosphere challenge with approximately 95% and 91% reductions in cystic metacestode and total metacestode recoveries, respectively. Rats vaccinated with fusion proteins of family 1 produced antibodies which reacted with a 21-kDa oncosphere antigen component which appeared to be a major oncosphere stage-specific antigen. |
| Databáze: | OpenAIRE |
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