Multiplexed Profiling of Single Extracellular Vesicles
| Autor: | Hakho Lee, Xandra O. Breakefield, Ralph Weissleder, Leonora Balaj, Kyungheon Lee, Kyle B. Fraser, Bassel Ghaddar, Eunha Kim, E. Antonio Chiocca, Katy Yang |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
General Physics and Astronomy Tumor cells Extracellular vesicles Article 03 medical and health sciences Extracellular Vesicles 0302 clinical medicine Cell Line Tumor Lab-On-A-Chip Devices medicine Biomarkers Tumor Humans General Materials Science Vesicle Optical Imaging General Engineering Extracellular vesicle Equipment Design medicine.disease Microvesicles 030104 developmental biology 030220 oncology & carcinogenesis Biophysics Glioblastoma |
| Popis: | Extracellular vesicles (EV) are a family of cell-originating, membrane-enveloped nanoparticles with diverse biological function, diagnostic potential, and therapeutic applications. While EV can be abundant in circulation, their small size (∼4 order of magnitude smaller than cells) has necessitated bulk analyses, making many more nuanced biological explorations, cell of origin questions, or heterogeneity investigations impossible. Here we describe a single EV analysis (SEA) technique which is simple, sensitive, multiplexable, and practical. We profiled glioblastoma EV and discovered surprising variations in putative pan-EV as well as tumor cell markers on EV. These analyses shed light on the heterogeneous biomarker profiles of EV. The SEA technology has the potential to address fundamental questions in vesicle biology and clinical applications. |
| Databáze: | OpenAIRE |
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