Sonic hedgehog expression correlates with distant metastasis in pancreatic adenocarcinoma
| Autor: | Juntao Dai, Yilong Du, Guorong Chen, Kaixing Ai |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Oncology
Adult Male medicine.medical_specialty China animal structures Endocrinology Diabetes and Metabolism Biology Adenocarcinoma medicine.disease_cause Endocrinology Internal medicine Pancreatic cancer Internal Medicine medicine Sonic Hedgehog Protein Biomarkers Tumor Humans Hedgehog Proteins Neoplasm Invasiveness RNA Messenger Sonic hedgehog Aged Neoplasm Staging Aged 80 and over Messenger RNA Chi-Square Distribution Hepatology Reverse Transcriptase Polymerase Chain Reaction Middle Aged medicine.disease Immunohistochemistry Up-Regulation Pancreatic Neoplasms medicine.anatomical_structure embryonic structures Cancer research biology.protein Female Carcinogenesis Pancreas |
| Zdroj: | Pancreas. 40(2) |
| ISSN: | 1536-4828 |
| Popis: | OBJECTIVES To investigate the expression and clinical significance of Sonic hedgehog (Shh) in pancreatic adenocarcinoma. METHODS The expression of Shh protein was examined in 34 surgical specimens of primary pancreatic adenocarcinoma, 21 nonmalignant specimens of the pancreas by immunohistochemistry streptavidin-perosidase (SP) method. In addition, semiquantitative reverse transcriptase polymerase chain reaction was carried out to analyze Shh mRNA expression in 22 pairs of freshly resected pancreatic adenocarcinoma tissues and their adjacent nontumorous tissues. RESULTS The positive expression rate of Shh protein was 64.7% (22/34) in 34 surgical specimens of primary pancreatic adenocarcinoma and 0% (0/21) in 21 nonmalignant specimens of the pancreas. The expression rate of Shh was higher in pancreatic adenocarcinoma tissues than that of nonmalignant pancreatic tissues (χ2 = 22.647, P = 0.000). Sonic hedgehog protein expression correlated with TNM stages and distant metastasis. Moreover, the expression levels of Shh mRNA were higher in pancreatic adenocarcinoma tissues than that of the matched adjacent nontumorous tissues. CONCLUSIONS Sonic hedgehog might play a pivotal role during tumorigenesis of pancreatic adenocarcinoma, and high Shh expression might be associated with the malignant potential of pancreatic cancer. |
| Databáze: | OpenAIRE |
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