Direct affinity screening chromatography–mass spectrometry assay for identification of antibacterial agents from natural product sources
| Autor: | Evelyn H. Wang, Stephanie M.C. Smith, Sunaina Rao, Kevin A. Schug, Sijia Shen, Laura D. Mydlarz, Laura R. Hunt |
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| Rok vydání: | 2012 |
| Předmět: |
Flow injection analysis
Biological Products Spectrometry Mass Electrospray Ionization Electrospray Alanine Natural product Chromatography Drug discovery Lysine Electrospray ionization Anthozoa Gram-Positive Bacteria Mass spectrometry Biochemistry Chromatography Affinity Anti-Bacterial Agents Analytical Chemistry chemistry.chemical_compound chemistry Animals Environmental Chemistry Titration Oligopeptides Spectroscopy |
| Zdroj: | Analytica Chimica Acta. 713:103-110 |
| ISSN: | 0003-2670 |
| Popis: | A direct affinity screening – mass spectrometry assay, coupled to liquid chromatography, is presented as a tool for natural product drug discovery. Using the assay, fractionated extracts from a Caribbean gorgonian coral were shown to contain a new chemical entity (NCE) which binds to a mimic of the Gram positive bacterial cell wall (lysine– d -alanine– d -alanine). Conditions for observation of a specific noncovalent complex between the NCE and the target mimic using electrospray ionization-mass spectrometry were validated in a series of positive and negative control experiments, which featured flow injection analysis-based titrations. While the structural identity of the NCE could not be determined due to limited sample quantities, this work provides proof-of-principle for such an approach to potentially accelerate drug discovery from natural product sources. |
| Databáze: | OpenAIRE |
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