BRCA mutations cause reduction in miR-200c expression in triple negative breast cancer
| Autor: | Ismet Tasdelen, Gulsah Cecener, Gulcin Tezcan, Unal Egeli, Berrin Tunca, Elif Erturk, Sahsine Tolunay, Sehsuvar Gokgoz |
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| Přispěvatelé: | Uludağ Üniversitesi/Sağlık Meslek Yükseokulu/Tıbbi Laboratuvar Teknikleri Programı., Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyoloji Anabilim Dalı., Uludağ Üniversitesi/Tıp Fakültesi/Genel Cerrahi Anabilim Dalı., Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Patoloji Anabilim Dalı., Ertürk, Elif, Çeçener, Gülşah, Tezcan, Gülçin, Egeli, Ünal, Tunca, Berrin, Gökgöz, Şehsuvar, Tolunay, Şahsine, Taşdelen, İsmet, AAK-3371-2021, AAP-9988-2020, AAH-3843-2020, AAH-1420-2021, ABI-6078-2020, EXK-4525-2022, AAI-1612-2021, EBN-1186-2022 |
| Rok vydání: | 2015 |
| Předmět: |
Vascular Endothelial Growth Factor A
Mırn200 microrna human Resistance Triple Negative Breast Neoplasms Bioinformatics Subclass Metastasis Cancer growth Pathology Mirnlet7 microRNA human Triple negative breast cancer Disease course skin and connective tissue diseases Down-regulation Triple-negative breast cancer Zeb1 Gene expression regulation Genetics & Heredity Progression BRCA1 Protein Circulating Microrna Stomach Neoplasms Breast Neoplasms MicroRNA General Medicine Middle Aged Epithelial-mesenchymal transition MicroRNA-200c Gene expression profiling Gene Expression Regulation Neoplastic Vascular endothelial growth factor A Reverse transcription polymerase chain reaction Disease Progression Female BRCA1/2 gene mutations Feedback loop Human Adult Clinical article Tumor invasion Vasculotropin A Ovarian-cancer MiR-200c Biology Article Breast cancer VEGFA protein human microRNA Genetics medicine Humans Family Gene mutation Human tissue Oncogene Loss function BRCA1 protein human BRCA2 Protein Neoplastic BRCA mutation medicine.disease BRCA2 protein human Let 7a gene MicroRNAs VEGFA gene Mutation Cancer research Controlled study |
| Zdroj: | Gene. 556:163-169 |
| ISSN: | 0378-1119 |
| Popis: | Triple negative breast cancer (TNBC) is the most aggressive and poorly understood subclass of breast cancer (BC). Over the recent years, miRNA expression studies have been providing certain detailed overview that aberrant expression of miRNAs is associated with TNBC. Although TNBC tumors are strongly connected with loss of function of BRCA genes, there is no knowledge about the effect of BRCA mutation status on miRNA expressions in TNBC cases. The aims of this study were to evaluate the expression profile of miRNAs that plays role in TNBC progression and the role of BRCA mutations in their regulation. The expression level of BC associated 13 miRNAs was analyzed in 7 BRCA mutations positive, 6 BRCA mutations negative TNBC cases and 20 non-tumoral tissues using RT-PCR. According to RT2 Profiler PCR Array Data Analysis, let-7a expression was 4.67 fold reduced in TNBCs as compared to normal tissues (P = 0.031). In addition, miR-200c expression was 5.75 fold reduced in BRCA mutation positive TNBC tumors (P = 0.005). Analysis revealed a negative correlation between miR-200c and VEGFA expressions (r = -468). Thus, miR-200c may be involved in invasion and metastasis in TNBC cases with BRCA mutation. In this study we provide the knowledge on the first report of association between microRNA-200c and BRCA mutations in TNBC. Further studies and evaluations are required, but this miRNA may provide novel therapeutic molecular targets for TNBC treatment and new directions for the development of anticancer drugs. |
| Databáze: | OpenAIRE |
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