Efficient production of nonactin by Streptomyces griseus subsp. griseus
| Autor: | Shaolun Zheng, Yulian Zhan |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Polymers 030106 microbiology Immunology Ionophore Nonactin Applied Microbiology and Biotechnology Microbiology Chemical synthesis Streptomyces 03 medical and health sciences chemistry.chemical_compound Biosynthesis Genetics Ammonium Molecular Biology Chromatography biology Streptomyces griseus General Medicine biology.organism_classification Anti-Bacterial Agents 030104 developmental biology chemistry Biochemistry Fermentation Macrolides |
| Zdroj: | Canadian Journal of Microbiology. 62:711-714 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/cjm-2016-0248 |
| Popis: | Here we report the production of the cyclic macrotetrolide nonactin from the fermentation culture of Streptomyces griseus subsp. griseus. Nonactin is a member of a family of naturally occurring cyclic ionophores known as the macrotetrolide antibiotics. Our fermentation procedure of Streptomyces griseus was performed at 30 °C and 200 rev·min−1 for 5 days on a rotary shaker. Diaion HP-20 and Amberlite XAD-16 were added to the fermentation medium. Isolated yield of nonactin was up to 80 mg·L−1 using our methodology. Nonactin is commonly known as an ammonium ionophore and also exhibits antibacterial, antiviral, and antitumor activities. It is also widely used for the preparation of ion-selective electrodes and sensors. Chemical synthesis of nonactin has been achieved by some groups; however, overall yields are very low, making efficient biosynthesis an attractive means of production. |
| Databáze: | OpenAIRE |
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