Paired Ductal CarcinomaIn Situand Invasive Breast Cancer Lesions in the D-Loop of the Mitochondrial Genome Indicate a Cancerization Field Effect
| Autor: | K Gehman, MK Froberg, T Gehman, Andrew Harbottle, H Brown, Andrea Maggrah, Ryan Parr, Jennifer Creed, Kerry Robinson, D Klein, Roy Wittock, Brian Reguly |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Article Subject Ductal lavage medicine.medical_treatment lcsh:Medicine Apoptosis Breast Neoplasms Biology General Biochemistry Genetics and Molecular Biology Breast cancer Nipple Aspirate Fluid Biopsy Biomarkers Tumor medicine Humans skin and connective tissue diseases Chromatography High Pressure Liquid Aged DNA Primers General Immunology and Microbiology medicine.diagnostic_test lcsh:R Carcinoma Ductal Breast Lumpectomy General Medicine Middle Aged Ductal carcinoma medicine.disease Heteroplasmy Carcinoma Intraductal Noninfiltrating Genome Mitochondrial Mutation Female Mastectomy Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2013 (2013) |
| ISSN: | 2314-6141 2314-6133 |
| DOI: | 10.1155/2013/379438 |
| Popis: | Alterations in the mitochondrial genome have been chronicled in most solid tumors, including breast cancer. The intent of this paper is to compare and document somatic mitochondrial D-loop mutations in paired samples of ductal carcinomain situ(DCIS) and invasive breast cancer (IBC) indicating a potential breast ductal epithelial cancerization field effect. Paired samples of these histopathologies were laser-captured microdissected (LCM) from biopsy, lumpectomy, and mastectomy tissues. Blood samples were collected as germplasm control references. For each patient, hypervariable region 1 (HV1) in the D-loop portion of the mitochondrial genome (mtGenome) was sequenced for all 3 clinical samples. Specific parallel somatic heteroplasmic alterations between these histopathologies, particularly at sites 16189, 16223, 16224, 16270, and 16291, suggest the presence of an epithelial, mitochondrial cancerization field effect. These results indicate that further characterization of the mutational pathway of DCIS and IBC may help establish the invasive potential of DCIS. Moreover, this paper indicates that biofluids with low cellularity, such as nipple aspirate fluid and/or ductal lavage, warrant further investigation as early and minimally invasive detection mediums of a cancerization field effect within breast tissue. |
| Databáze: | OpenAIRE |
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