Aortic Aneurysms in a Rat Model: In Vivo MR Imaging of Endovascular Cell Therapy
| Autor: | Jean-Noel Pons, Philippe Meric, Charlotte Rivière, Didier Letourneur, Jean-François Deux, Jacky Roger, F. Boudghene, Jiangping Dai, Brigitte Gillet, Eric Allaire, Florence Gazeau |
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| Přispěvatelé: | Matière et Systèmes Complexes (MSC (UMR_7057)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Sauvant, Nicole, Thérapeutiques substitutives du coeur et des vaisseaux (TSCV), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), CNRS UMR 7057 - Laboratoire Matières et Systèmes Complexes (MSC) (MSC), Centre National de la Recherche Scientifique (CNRS), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Male
Vascular smooth muscle Cell Transplantation Contrast Media 030204 cardiovascular system & hematology Muscle Smooth Vascular MESH: Magnetic Resonance Imaging Cell therapy Aortic aneurysm 0302 clinical medicine MESH: Animals ComputingMilieux_MISCELLANEOUS 0303 health sciences medicine.diagnostic_test [SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph] MESH: Muscle Smooth Vascular Magnetic Resonance Imaging MESH: Cell Transplantation cardiovascular system Radiology MESH: Aortic Aneurysm Abdominal medicine.medical_specialty MESH: Rats [SDV.BC]Life Sciences [q-bio]/Cellular Biology Injections 03 medical and health sciences In vivo MESH: Contrast Media medicine Animals MESH: Injections Radiology Nuclear Medicine and imaging [PHYS.MECA.BIOM]Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph] [SDV.BC] Life Sciences [q-bio]/Cellular Biology 030304 developmental biology MESH: Rats Inbred F344 business.industry [PHYS.MECA.BIOM] Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph] Institutional Animal Care and Use Committee Magnetic resonance imaging medicine.disease Mr imaging Ferrosoferric Oxide Rats Inbred F344 MESH: Male Rats Radiography Disease Models Animal MESH: Ferrosoferric Oxide Nanoparticles MESH: Disease Models Animal business Nuclear medicine MESH: Nanoparticles Ex vivo Aortic Aneurysm Abdominal |
| Zdroj: | Radiology Radiology, Radiological Society of North America, 2008, 246, pp.185-192 Radiology, Radiological Society of North America, 2008, 246 ((1)), pp.185-92 Radiology, Radiological Society of North America, 2008, 246 (1), pp.185-92. ⟨10.1148/radiol.2461070032⟩ |
| ISSN: | 0033-8419 1527-1315 |
| Popis: | International audience; PURPOSE: To prospectively evaluate in rats whether magnetic cell labeling can be used to noninvasively assess the technical success of endovascular cell therapy for abdominal aortic aneurysms (AAAs). MATERIALS AND METHODS: The study was approved by an institutional animal care and use committee. Vascular smooth muscle cells (VSMCs) labeled with iron oxide nanoparticles were seeded endovascularly in already formed AAAs. T2*-weighted gradient-echo and T2-weighted spin-echo magnetic resonance (MR) imaging was performed in vivo at 1.5 T before and 30 minutes after the injection of iron-loaded VSMCs (14 rats), nonlabeled VSMCs (three rats), or iron-free particles (three rats). Ten rats were euthanized shortly after the injection (day 0). Of the 10 remaining rats, which were seeded with iron-loaded cells, three were imaged on day 7 after cell delivery; three, on day 14; and four, on day 28; then they were euthanized. Ex vivo high-field-strength MR imaging of two AAAs was performed 28 days after cell delivery. Histologic examination of cross sections of all AAAs was performed. Statistical evaluations were performed with a nonparametric Wilcoxon correlation test. RESULTS: Magnetic cell labeling did not alter the capability of VSMCs to stabilize the diameter of the aneurysms. T2*-weighted gradient-echo images showed areas of hypointense signal within the aortic wall immediately and up to 1 month after cell therapy. The mean signal intensity decreased significantly after cell delivery (from 2362 +/- 244 [standard deviation] before to 434 +/- 275 after delivery, P < .001). Areas of hypointense signal and iron-loaded VSMCs were colocalized in the area of aortic wall reconstruction at both high-field-strength MR imaging and histologic analysis. CONCLUSION: MR imaging with magnetic cell labeling can be used to document endovascular cell delivery in already formed AAAs in rats. |
| Databáze: | OpenAIRE |
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