Epigallocatechin gallate protects against homocysteine-induced vascular smooth muscle cell proliferation
| Autor: | Xiu Hong Yang, Xiao Li Zhan, Yan Hong Gu, Li Li Guo, Hui Min Jin |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Vascular smooth muscle MAP Kinase Signaling System p38 mitogen-activated protein kinases Clinical Biochemistry Myocytes Smooth Muscle Muscle Proteins Epigallocatechin gallate Biology Catechin Muscle Smooth Vascular Cell Line 03 medical and health sciences chemistry.chemical_compound Internal medicine medicine Humans Receptor Protein kinase A Molecular Biology Homocysteine Cell Proliferation Kinase Cell Biology General Medicine Angiotensin II 030104 developmental biology Endocrinology chemistry Signal transduction |
| Zdroj: | Molecular and cellular biochemistry. 439(1-2) |
| ISSN: | 1573-4919 |
| Popis: | Epigallocatechin gallate (EGCG), a bioactive ingredient of green tea, plays a protective role in the cardiovascular system. Homocysteine (Hcy) is a major risk factor for chronic kidney disease and cardiovascular disease. The present study aimed to investigate the role of EGCG in Hcy-induced proliferation of vascular smooth muscle cells (VSMCs) and its underlying mechanism. We also explored the roles of rennin-angiotensin system (RAS), extracellular signal-regulated kinases (ERK1/2), and p38 mitogen-activated protein kinase (p38 MAPK) in this process. Human aortic smooth muscle cells (HASMCs) were treated with different drugs for different periods. The proliferation rate of HASMCs was detected using the CCK-8 and BrdU labeling assays. The Western blot assay was used to determine the expression levels of angiotensin II type 1 receptor (AT-1R), ERK1/2, and p38 MAPK. Compared with the control group, the HASMCs treated with Hcy at different doses (100, 200, 500, and 1000 µM) showed significantly increased proliferation. Hcy increased the expression of AT-1R, whereas EGCG decreased the protein expression of AT-1R. Furthermore, we found that Hcy-induced expression of p-ERK1/2 and p-p38MAPK was dependent on AT-1R. Compared with Hcy (500 µM)-treated cells, EGCG (20 µM)-treated cells showed decreased proliferation as well as expression of AT-1R, p-ERK1/2, and p-p38MAPK. In addition, HASMC proliferation was suppressed by the addition of an AT-1R blocker (olmesartan), an ERK1/2 inhibitor (PD98059), and a p38MAPK inhibitor (SB202190). EGCG can inhibit AT-1R and affect ERK1/2 and p38MAPK signaling pathways, resulting in the decrease of VSMC proliferation induced by Hcy. |
| Databáze: | OpenAIRE |
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