Quantitative Release of Live Microorganisms from Infected Macrophages by Sodium Dodecyl Sulphate
| Autor: | R Behin, Biroum-Noerjasin, J Mauel |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Leishmania
Time Factors biology Host (biology) Chemistry Macrophages Microorganism Sodium Guinea Pigs Inorganic chemistry Sodium Dodecyl Sulfate chemistry.chemical_element General Medicine biology.organism_classification medicine.disease_cause General Biochemistry Genetics and Molecular Biology Microbiology Surface-Active Agents Listeria monocytogenes Methods medicine Animals Fatty Alcohols Bacteria |
| Zdroj: | Nature New Biology. 244:93-94 |
| ISSN: | 2058-1092 0090-0028 |
| DOI: | 10.1038/newbio244093a0 |
| Popis: | RELEASE of viable microorganisms from host cells is a necessary step in many studies of host-parasite relationships. Techniques developed include hypotonic1 or hypobaric shock2 and sonication3 and the use of chemical reagents4, but none of the available methods is of general application. We describe here a simple technique for obtaining quantitative release of Leishmania parasites from infected macrophages. This method was also successful in releasing another microorganism, the bacterium Listeria monocytogenes, from infected cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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