Transfer of T-cell-mediated, antigen-specific delayed type hypersensitivity reactions to naive recipient inbred pigs
| Autor: | S. T. Licence, Anthony Whyte, Richard M. Binns |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Inflammation
Male General Veterinary Chemistry Cell adhesion molecule Swine T cell T-Lymphocytes Tuberculin Immunotherapy Adoptive Endothelial activation medicine.anatomical_structure Antigen specific Lymphocyte Transfusion Immunology medicine Local infiltration Animals Dinitrofluorobenzene Female Hypersensitivity Delayed medicine.symptom Major histocompatibility |
| Zdroj: | Research in veterinary science. 60(1) |
| ISSN: | 0034-5288 |
| Popis: | Using inbred major histocompatibility complex-homozygous sla b/b pigs, delayed-type hypersensitivity ( dth ) reactions against either intradermal tuberculin ( ppd ) or topical 2,4-dinitro-l-fluorobenzene ( dnfb ) were transferred specifically by the intravenous injection of approximately 6 × 10 8 blood lymphocytes kg −1 bodyweight from donors sensitised, respectively, either with bcg or with dnfb into three-week-old piglets from an inbred litter. This antigen-specific, passively acquired sensitivity was revealed by three measures of DTH reactivity: first, macroscopic inflammation, which developed at the rate and intensity expected for actively acquired sensitivity to DNFB or PPD in older pigs; secondly, similarly enhanced local specific uptake of intravenously injected 51 Cr-labelled normal lymphocytes (more than 35-fold for each); and, thirdly, histological evidence of markedly increased local infiltration of CD45 + lymphocytes and polymorphs, endothelial activation and the expression of adhesion molecules. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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