Polymerase chain reaction for detection of Mycoplasma gallisepticum in environmental samples
| Autor: | Isabelle Kempf, Corinne Marois, Fabienne Dufour-Gesbert |
|---|---|
| Přispěvatelé: | Laboratoire d'études et de recherches avicoles, porcines et piscicoles, Agence Française de Sécurité Sanitaire des Aliments (AFSSA) |
| Rok vydání: | 2002 |
| Předmět: |
Mycoplasma gallisepticum
Spiroplasma MESH: Poultry Diseases medicine.disease_cause MESH: Dust Polymerase Chain Reaction Microbiology law.invention Ureaplasma MESH: Mycoplasma Feces Mycoplasma Food Animals Species Specificity law Water Supply medicine Food microbiology MESH: Species Specificity Animals MESH: Animals Mycoplasma Infections MESH: Feathers MESH: Water Supply MESH: Food Microbiology Polymerase chain reaction Poultry Diseases General Immunology and Microbiology biology MESH: Feces MESH: Polymerase Chain Reaction Dust MESH: Mycoplasma Infections Feathers biology.organism_classification MESH: Housing Animal [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Housing Animal MESH: Water Microbiology Acholeplasma Feather visual_art visual_art.visual_art_medium Food Microbiology Animal Science and Zoology Water Microbiology |
| Zdroj: | Avian Pathology Avian Pathology, Taylor & Francis, 2002, 31 (2), pp.163-8. ⟨10.1080/03079450120118658⟩ |
| ISSN: | 0307-9457 1465-3338 |
| DOI: | 10.1080/03079450120118658⟩ |
| Popis: | The polymerase chain reaction (PCR) was used to detect Mycoplasma gallisepticum in samples collected from the environment of experimentally or naturally infected poultry. Culture was also used in the experimental infections. Of 160 samples of food, drinking water, feathers, droppings or dust collected during experimental infection, 103 were positive using a M. gallisepticum-specific PCR (MG-PCR) and 68 were positive using a PCR (mycoplasma-PCR) that detects all species of the genera Mycoplasma, Spiroplasma, Acholeplasma and Ureaplasma. Six of these samples were also positive by culture. In environmental samples collected on a depopulated M. gallisepticum-positive turkey farm, three and two out of a total of 12 were positive by mycoplasma-PCR and MG-PCR, respectively. These results indicate the disseminating capacity of this mycoplasma and the possible use of PCR methods for epidemiological analyses and control of farm decontamination before the introduction of new birds. |
| Databáze: | OpenAIRE |
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