Gas chromatographic/mass spectrometric quantitative analysis of eicosanoids in human oesophageal mucosa
| Autor: | Ottignon Y, M. M. Toubin, C. Moussard, D. Alber, J.C. Henry, J P Deschamps |
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| Rok vydání: | 1988 |
| Předmět: |
Chromatography
Mucous Membrane biology Ether Biochemistry In vitro Gas Chromatography-Mass Spectrometry Lipoxygenase chemistry.chemical_compound Esophagus chemistry Biosynthesis Eicosanoic Acids biology.protein Prostaglandins Molecular Medicine Humans lipids (amino acids peptides and proteins) Selected ion monitoring Cyclooxygenase Gas chromatography–mass spectrometry Hydroxy Acids Quantitative analysis (chemistry) Spectroscopy |
| Zdroj: | Biomedicalenvironmental mass spectrometry. 16(1-12) |
| ISSN: | 0887-6134 |
| Popis: | In order to investigate the contribution of eicosanoids to human oesophageal functions and disorders (gastrooesophageal reflux, GOR and reflux oesophagitis, RO), we have used a selected ion monitoring gas chromatographic/mass spectrometric methodology to quantify the cyclooxygenase and lipoxygenase products biosynthesized in vitro by endoscopic mucosal biopsy specimens. Prostaglandins (PGs) were quantified as MEMOTMS derivatives and HETEs, as hydrogenated methyl ester of tert-butyldimethylsilyl) ether derivatives. PGE2, PGF2 alpha appeared as the major prostanoids, whereas 12HETE seemed to be the major lipoxygenase product. In the case of GOR or RO, biosynthesis of PGE2 was dramatically increased, while no change could be detected for 12HETE. PGE2 increase seems to be related to inflammatory reaction, in which its exact role remains unclear. Moreover, it cannot be excluded that PGE2 is a side product which might be protective to the oesophageal mucosa. |
| Databáze: | OpenAIRE |
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