Immunophilin-like FKBP42/TWISTED DWARF1 Interacts with the Receptor Kinase BRI1 to Regulate Brassinosteroid Signaling in Arabidopsis
| Autor: | Juthamas Chaiwanon, Ying Sun, Veder J. Garcia, Heather N. Cartwright, Zhi-Yong Wang |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Mutant Arabidopsis Plant Science Protein Serine-Threonine Kinases Bioinformatics Tropomyosin receptor kinase C Article Tacrolimus Binding Proteins 03 medical and health sciences chemistry.chemical_compound Protein Domains Brassinosteroids Brassinosteroid Receptor Molecular Biology Transcription factor biology Kinase Arabidopsis Proteins fungi Cell Membrane biology.organism_classification Cell biology Protein Transport 030104 developmental biology chemistry Mutation Signal transduction Protein Kinases Protein Binding Signal Transduction |
| Zdroj: | Molecular plant. 9(4) |
| ISSN: | 1752-9867 |
| Popis: | Mutation of the immunophilin-like FK506-binding protein TWISTED DWARF1 (FKBP42/TWD1) causes dwarf and twisted-organ phenotypes in Arabidopsis. However, the function of FKBP42 is not fully understood at the molecular level. Using genetic, physiological, and immunological experiments, we show here that FKBP42/TWD1 is necessary for brassinosteroid (BR) signal transduction. The twd1 mutant showed reduced BR sensitivity in growth responses and activation of the BZR1 transcription factor. However, twd1 showed normal responses to an inhibitor of BIN2/GSK3, suggesting that twd1 has a defect upstream of BIN2 in the BR signaling pathway. In vitro and in vivo assays showed that TWD1 interacts physically with the kinase domains of the BR receptor kinases BRI1 and BAK1. TWD1 is not required for normal localization of BRI1-GFP to the plasma membrane or for activation of the flagellin receptor kinase FLS2. Our results suggest that FKBP42/TWD1 plays a specific role in the activation of BRI1 receptor kinase. |
| Databáze: | OpenAIRE |
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