Measuring cell displacements in opaque tissues: dynamic light scattering in the multiple scattering regime
| Autor: | Romain Pierrat, Vincent Levy, Benjamin Brunel, Arnaud Millet, Antoine Delon, Monika E. Dolega, Giovanni Cappello |
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| Přispěvatelé: | Cappello, Giovanni, Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] (LIPhy ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Equipe U1205 Inserm 'Mechano-biology, Immunity and Cancer', BrainTech Laboratory [CHU Grenoble Alpes - Inserm U1205] (Brain Tech Lab ), CHU Grenoble-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-CHU Grenoble-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut Langevin - Ondes et Images (UMR7587) (IL), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Paris (UP)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
[PHYS.PHYS.PHYS-OPTICS] Physics [physics]/Physics [physics]/Optics [physics.optics]
Opacity Forward scatter [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] 01 natural sciences Article Quantitative Biology::Cell Behavior 010309 optics 03 medical and health sciences Optics Dynamic light scattering Optical coherence tomography 0103 physical sciences medicine Diffusion (business) Spectroscopy 030304 developmental biology Physics 0303 health sciences [PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics] medicine.diagnostic_test [PHYS.PHYS.PHYS-BIO-PH] Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] Scattering business.industry Atomic and Molecular Physics and Optics Millimeter business Biotechnology |
| Zdroj: | Biomedical optics express Biomedical optics express, 2020, 11 (4), pp.2277. ⟨10.1364/BOE.388360⟩ Biomedical optics express, Optical Society of America-OSA Publishing, 2020, 11 (4), pp.2277. ⟨10.1364/BOE.388360⟩ |
| ISSN: | 2156-7085 |
| DOI: | 10.1364/BOE.388360 |
| Popis: | International audience; Coherent light scattered by tissues brings structural and dynamic information, at depth, that standard imaging techniques cannot reach. Dynamics of cells or sub-cellular elements can be measured thanks to dynamic light scattering in thin samples (single scattering regime) or thanks to diffusive wave spectroscopy in thick samples (diffusion regime). Here, we address the intermediate regime and provide an analytical relationship between scattered light fluctuations and the distribution of cell displacements as a function of time. We illustrate our method by characterizing cell motility inside half millimeter thick multicellular aggregates. |
| Databáze: | OpenAIRE |
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