Experimental infection in gerbils by Conidiobolus lamprauges
| Autor: | Luciano Nakazato, Camila Gonçalves de Campos, Stéfhano Luis Cândido, Isabela de Godoy, João Vitor Amorim Galceran, Caroline Argenta Pescador, Valéria Dutra |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Nasal cavity Pathology medicine.medical_specialty Cyclophosphamide 040301 veterinary sciences medicine.medical_treatment 030106 microbiology Hyphae Sheep Diseases Biology Microbiology Polymerase Chain Reaction 0403 veterinary science Pathogenesis 03 medical and health sciences Immunocompromised Host Random Allocation Zygomycosis medicine Animals Conidiobolus Lung Sheep Immunosuppression 04 agricultural and veterinary sciences Spores Fungal medicine.disease biology.organism_classification Disease Models Animal Infectious Diseases medicine.anatomical_structure Infectious disease (medical specialty) Conidiobolomycosis Immunology Entomophthoramycosis Nasal Cavity Gerbillinae medicine.drug |
| Zdroj: | Microbial pathogenesis. 105 |
| ISSN: | 1096-1208 |
| Popis: | Conidiobolomycosis is an emerging entomophthoramycosis caused by fungi Conidiobolus spp. Animal models are essential for the study of infectious disease in various areas such as pathogenesis, diagnostic methods, treatment and prevention. There is not currently an animal model for conidiobolomycosis. The aim of this study was to create an experimental infection protocol for Conidiobolus lamprauges in gerbils (Meriones unguiculatus). The study animals were randomly divided into four groups of four animals: immunosuppressed with cyclophosphamide (CPA) and infected with C. lamprauges (G1), immunocompetent and infected with C. lamprauges (G2), immunosuppressed with CPA (G3), and an immunocompetent control group (G4). Clinical signs were observed only in G1 animals, where the mortality rate reached 75% by day 7 after infection (AI) with a median survival of 2 days. C. lamprauges was detected only in G1, both by PCR and by isolation. Necropsies of the G1 animals showed lesions in the nasal cavity and lung tissue. These lesions were characterized by polymorphonuclear infiltrate cells and by the presence of hyphal structures under silver staining. This animal model will be useful for further investigation of diseases caused by C. lamprauges, particularly of those associated with immunosuppression factors in naturally occurring animal infections. |
| Databáze: | OpenAIRE |
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