Human T-cell-leukemia virus type I in post-transfusional spastic paraparesis: Complete proviral sequence from uncultured blood cells
| Autor: | Ali Bazarbachi, Menger Huang, Antoine Gessain, Fortuna Saal, Ali Saib, Jorge Peries, Hugues De The, Francis Galibert |
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| Rok vydání: | 1995 |
| Předmět: |
Adult
Male Cancer Research Genes Viral viruses Molecular Sequence Data Biology Virus Open Reading Frames Proviruses immune system diseases Sequence Homology Nucleic Acid hemic and lymphatic diseases Genotype Tropical spastic paraparesis medicine Humans Amino Acid Sequence Cloning Molecular Genetics Human T-lymphotropic virus 1 Base Sequence Sequence Homology Amino Acid Nucleic acid sequence Transfusion Reaction virus diseases Provirus medicine.disease Virology Paraparesis Tropical Spastic Alternative Splicing Leukemia Open reading frame Oncology Leukocytes Mononuclear Tissue tropism |
| Zdroj: | International Journal of Cancer. 63:494-499 |
| ISSN: | 1097-0215 0020-7136 |
| DOI: | 10.1002/ijc.2910630406 |
| Popis: | Human-T-cell-leukemia virus type I (HTLV-I) is the causative agent of adult T-cell leukemia/lymphoma (ATL) and tropical spastic paraparesis/ HTLV-I-associated myelopathy (TSP/ HAM). The different disease outcome may be attributable to subtle mutations leading to modification of viral tropism or infectivity. Initial attempts found a very high level of sequence conservation among all HTLV-I strains. However, only one complete proviral DNA sequence is reported from a TSP/HAM patient, with a provirus derived from immortalized lymphocytes, which might be expected to be a leukemogenic variant rather than a neutrotropic one. We cloned and sequenced a complete HTLV-I provirus (HTLV-1 boi ) derived from the uncultured lymphocytes of a sub-acute post-transfusional TSP/HAM patient with clonal integration of HTLV-I. HTLV-1 boi proviral genome is 9033 bp long, and its overall genetic organization is similar to that of the prototype HTLV-I(ATK), without major deletions or insertions. No premature termination codon was found in the 4 open reading frames of the pX region. Divergence at the nucleotide level of HTLV-1 Boi from the reported full-length HTLV-I varies from I to 9.4%, and indicates that it corresponds to a cosmopolitan genotype. This study did not identify specific sequences associated with neurotropic strains. |
| Databáze: | OpenAIRE |
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