Soluble Fas (FasB) regulates luteal cell apoptosis during luteolysis in murine ovaries
| Autor: | Munetake Shimabe, Hajime Miyamoto, Noboru Manabe, Kohji Komatsu, Minako Kiso |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
endocrine system
medicine.medical_specialty Fas Ligand Protein Cell Survival Luteolysis Gene Expression Apoptosis In situ hybridization Biology Luteal phase Fas ligand law.invention Andrology Interferon-gamma Mice Corpus Luteum law Luteal Cells Internal medicine Genetics medicine Animals RNA Messenger fas Receptor Cells Cultured reproductive and urinary physiology Mice Inbred ICR Messenger RNA Membrane Glycoproteins Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha urogenital system Ovary Cell Biology Recombinant Proteins Endocrinology Solubility Recombinant DNA Immunohistochemistry Female hormones hormone substitutes and hormone antagonists Developmental Biology |
| Zdroj: | Molecular Reproduction and Development. 65:345-352 |
| ISSN: | 1098-2795 1040-452X |
| DOI: | 10.1002/mrd.10312 |
| Popis: | During luteolysis, luteal cell apoptosis is induced by the Fas ligand (FasL)/Fas system. In murine luteal bodies, we demonstrated the expression of mRNA of soluble form of Fas (FasB), which binds to FasL and prevents apoptosis induction. By in situ hybridization, strong expression of FasB mRNA was observed in normal luteal bodies, in which no apoptotic cells were detected, but negative/trace expression in regressing luteal bodies, in which many apoptotic cells were observed. Immunohistochemical staining revealed that Fas and TNF-alpha were localized in both normal and regressing luteal bodies, but IFN-gamma was localized only in regressing luteal bodies. Apoptosis was induced in primary cultured luteal cells, when they were pretreated with TNF-alpha and IFN-gamma and then incubated with TNF-alpha, IFN-gamma, and mouse recombinant FasL (rFasL). However, no apoptosis was detected in the cells, when they were treated with rFasL alone, TNF-alpha alone, IFN-gamma alone, TNF-alpha and rFasL, IFN-gamma and rFasL, or TNF-alpha and IFN-gamma. Fas mRNA expression in cultured luteal cells was up-regulated by the treatment of TNF-alpha, IFN-gamma, or TNF-alpha and IFN-gamma. The expression of FasB mRNA was down-regulated, when the cells were treated with TNF-alpha and IFN-gamma, but its expression was not changed by the treatment of TNF-alpha alone or IFN-gamma alone. We conclude that FasB inhibits the apoptosis induction in luteal cells of normal luteal bodies, and that decreased FasB production induced by TNF-alpha and IFN-gamma made possible the apoptosis induction in the luteal cells of regressing luteal bodies. |
| Databáze: | OpenAIRE |
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