Structural characterization and screening of chemical markers of flavonoids in Lysimachiae Herba and Desmodii Styracifolii Herba by ultra high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry based metabolomics approach
Autor: | Caifeng Meng, Chunying Wang, Xueyan Fan, Xiaochen Liu, Ruina Liu, Xin Wang |
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Rok vydání: | 2019 |
Předmět: |
Clinical Biochemistry
Pharmaceutical Science Tandem mass spectrometry 01 natural sciences Analytical Chemistry Chemical marker Metabolomics Molecular level Tandem Mass Spectrometry Drug Discovery Medicine Chinese Traditional Quadrupole time of flight Data dependent Chromatography High Pressure Liquid Spectroscopy Primulaceae Flavonoids Chromatography Plant Extracts 010405 organic chemistry Chemistry 010401 analytical chemistry Fabaceae Chemical basis 0104 chemical sciences Multivariate Analysis Ultra high performance Biomarkers |
Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 171:52-64 |
ISSN: | 0731-7085 |
Popis: | In traditional Chinese medicine, Lysimachiae Herba (LH) and Desmodii Styracifolii Herba (DSH) have been widely used for the treatment of calculi, but there is a certain focus in clinical application. Flavonoids as their pharmacologically active substances were focusly studied to make clear of their chemical compositions and reveal the similarities and differences between LH and DHS by analysis of characteristic marker components at the molecular level. An ultra high performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF-MS/MS) approach based on metabolite profiling was established. The high-resolution data was acquired through data dependent acquisition (DDA) mode. Based on the targeted and untargeted analytical strategies, a total of 113 compounds were identified, of which 80 compounds existed in LH and 61 in DSH. Then multivariate statistical analysis was applied to further find the characteristic marker components, and a total number of 21 variables were screened as the valuable variables for discrimination. By matching with identified flavonoids, these 21 variables were corresponding to 15 flavonoids (including 6 from LH and 9 from DSH) which were firstly identified as the marker compounds. These results indicated that the UPLC-QTOF-MS/MS method with analysis strategy was a powerful tool for rapidly identification and screening of marker compounds of flavonoids between LH and DSH, and the 15 screened marker compounds provide a chemical basis for the further researches on the mechanisms of LH and DSH in the treatment of cholelithiasis and nephrolithiasis respectively. |
Databáze: | OpenAIRE |
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