The Hda1 histone deacetylase limits divergent non‐coding transcription and restricts transcription initiation frequency

Autor: Heta P. Patel, Niels A. Müller, Tineke L. Lenstra, Isabel Schwarz, Uthra Gowthaman, Desiré García-Pichardo, Sebastian Marquardt, Maxim Ivanov
Jazyk: angličtina
Předmět:
DYNAMICS
RNA
Untranslated
Saccharomyces cerevisiae Proteins
Transcription
Genetic
ACETYLATION
divergent non-coding (DNC) transcription
non‐coding RNA (ncRNA)
Repressor
RNA polymerase II
Saccharomyces cerevisiae
General Biochemistry
Genetics and Molecular Biology
Histone Deacetylases
Article
live-cell imaging
NOISE
Histones
Histone H3
Transcription (biology)
Gene Expression Regulation
Fungal
non-coding RNA (ncRNA)
REVEALS
BIDIRECTIONAL PROMOTERS
Promoter Regions
Genetic
RNA polymerase II transcription
Molecular Biology
GENE-EXPRESSION
live‐cell imaging
General Immunology and Microbiology
biology
ELONGATION
General Neuroscience
Promoter
Acetylation
Articles
FIDELITY
Cell biology
Nucleosomes
GENOME
Histone
Chromatin
Transcription & Genomics
biology.protein
Histone deacetylase complex
divergent non‐coding (DNC) transcription
RNA
Histone deacetylase
RNA Polymerase II
Zdroj: Gowthaman, U, Ivanov, M, Schwarz, I, Patel, H P, Müller, N A, Garcia-Pichardo, D, Lenstra, T L & Marquardt, S 2021, ' The Hda1 histone deacetylase limits divergent non-coding transcription and restricts transcription initiation frequency ', EMBO Journal, vol. 40, no. 23, 108903 . https://doi.org/10.15252/embj.2021108903
The EMBO Journal
ISSN: 1460-2075
0261-4189
DOI: 10.15252/embj.2021108903
Popis: Nucleosome‐depleted regions (NDRs) at gene promoters support initiation of RNA polymerase II transcription. Interestingly, transcription often initiates in both directions, resulting in an mRNA and a divergent non‐coding (DNC) transcript of unclear purpose. Here, we characterized the genetic architecture and molecular mechanism of DNC transcription in budding yeast. Using high‐throughput reverse genetic screens based on quantitative single‐cell fluorescence measurements, we identified the Hda1 histone deacetylase complex (Hda1C) as a repressor of DNC transcription. Nascent transcription profiling showed a genome‐wide role of Hda1C in repression of DNC transcription. Live‐cell imaging of transcription revealed that mutations in the Hda3 subunit increased the frequency of DNC transcription. Hda1C contributed to decreased acetylation of histone H3 in DNC transcription regions, supporting DNC transcription repression by histone deacetylation. Our data support the interpretation that DNC transcription results as a consequence of the NDR‐based architecture of eukaryotic promoters, but that it is governed by locus‐specific repression to maintain genome fidelity.
A yeast reverse‐genetic screen identifies factors affecting divergent non‐coding transcription.
Databáze: OpenAIRE
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