Uterine viability in the baboon after ligation of uterine vasculature: a pilot study to assess alternative perfusion and venous return for uterine transplantation
| Autor: | Stephen E. Zimberg, Benjamin Beran, M.L. Sprague, Tommaso Falcone, K. Rivas, Pedro F. Escobar, Marie E. Shockley, Katrin Arnolds, Andreas Tzakis |
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| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
Vaginoscopy Perfusion Imaging medicine.medical_treatment Uterus Pilot Projects Anastomosis Hysterectomy Veins Necrosis 03 medical and health sciences 0302 clinical medicine medicine.artery biology.animal medicine Animals Uterine artery Ligation Tissue Survival 030219 obstetrics & reproductive medicine biology business.industry Ovary Obstetrics and Gynecology Surgery Uterine Artery medicine.anatomical_structure Reproductive Medicine Regional Blood Flow 030220 oncology & carcinogenesis Models Animal cardiovascular system Female Papio hamadryas business Perfusion Baboon |
| Zdroj: | Fertility and Sterility. 107:1078-1082 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/j.fertnstert.2017.01.014 |
| Popis: | Objective To assess, in two separate groups of baboons, uterine viability after ligation of the uterine veins and uterine viability after ligation of both the uterine arteries and veins, respectively. Design Prospective, observational study. Setting Baboon breeding colony. Animal(s) Six naive female Papio hamadryas baboons with indicators of normal reproductive function. Intervention(s) Three baboons underwent surgical interruption of the uterine veins bilaterally, and three baboons underwent surgical interruption of the uterine arteries and the uterine veins bilaterally. All baboons also underwent colpotomy, cervico-vaginal reanastomosis, and intraoperative near-infrared fluorescence imaging after vessel ligation. In the postoperative period, transabdominal sonography, vaginoscopy, and endocervical biopsy were performed on all animals. Main Outcome Measure(s) Postoperative uterine and ovarian viability. Result(s) Near-infrared imaging confirmed intraoperative perfusion of the uterus and cervico-vaginal anastomosis in all cases. In all subjects, sonography revealed normal uteri, and vaginoscopy revealed well-healed anastomoses. Endocervical biopsies (five of six) demonstrated pathologically normal endocervical tissue without evidence of necrosis. Cyclical sex skin turgescence and menstruation were unanimously observed. Conclusion(s) Disruption of bilateral uterine vessels does not affect uterine or ovarian viability in the baboon. Bilateral uterine artery and vein ligation furthers development of a minimally invasive approach to donor hysterectomy. |
| Databáze: | OpenAIRE |
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